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800 pg/ml 锛�6铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 鍘熷�嶆績搴︿笉鐢ㄧ█閲嬬洿鎺ュ姞鍏�50ul銆�
400 pg/ml 锛�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨勫師鍊嶆(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
200 pg/ml 锛�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
100 pg/ml 锛�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
50 pg/ml 锛�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
25 pg/ml 锛�1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
0 pg/ml 锛堢┖鐧藉皪(du矛)鐓э級 鍘熷婵冨害涓嶇敤绋�閲嬬洿鎺ュ姞鍏�50ul銆�2锛� 娲楁粚绶╂矕娑诧紙50×锛夌殑绋�閲嬶細钂搁ぞ姘�50鍊嶇█閲�銆�
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2锛� 鏍规摎(j霉)寰呮脯妯ｅ搧鏁�(sh霉)閲忓姞涓婃(bi膩o)婧�(zh菙n)鍝佺殑鏁�(sh霉)閲忔焙瀹氭墍闇�鐨勬澘姊濇暩(sh霉)銆傛瘡鍊�(g猫)妯�(bi膩o)婧�(zh菙n)鍝佸拰绌虹櫧瀛斿缓璀板仛寰�(f霉)瀛斻�傛瘡鍊�(g猫)妯ｅ搧鏍规摎(j霉)鑷繁鐨勬暩(sh霉)閲忎締瀹�锛岃兘浣跨敤寰�(f霉)瀛旂殑鐩￠噺鍋氬京(f霉)瀛�銆�
3锛� 鍔犲叆绋�閲嬪ソ鍚庣殑妯�(bi膩o)婧�(zh菙n)鍝�50ul浜庡弽鎳�(y墨ng)瀛斻�佸姞鍏ュ緟娓ǎ鍝�50ul浜庡弽鎳�(y墨ng)瀛斿収(n猫i)銆傜珛鍗冲姞鍏�50ul鐨勭敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨銆傝搵涓婅啘鏉�锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�1灏忔檪(sh铆)銆�
4锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉�锛岀敥鍘绘礂婊屾恫锛岀敤鍚告按绱欐媿骞�銆傞噸寰�(f霉)姝ゆ搷浣�3娆�銆傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆�銆�
5锛� 姣忓瓟鍔犲叆80ul鐨勮Κ鍜岄張閰剁礌-HRP锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�30鍒嗛悩銆�
6锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉�锛岀敥鍘绘礂婊屾恫锛岀敤鍚告按绱欐媿骞�銆傞噸寰�(f霉)姝ゆ搷浣�3娆�銆傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆°��
7锛� 姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50ul锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�10鍒嗛悩銆傞伩鍏嶅厜鐓�銆�
8锛� 鍙栧嚭閰舵(bi膩o)鏉�锛岃繀閫熷姞鍏�50ul绲傛娑�锛屽姞鍏ョ祩姝㈡恫鍚庢噳(y墨ng)绔嬪嵆娓畾绲�(ji茅)鏋溿��
9锛� 鍦�450nm娉㈤暦铏曟脯瀹氬悇瀛旂殑OD鍊�銆�寤鸿浣跨敤鐨勫(sh铆)椹�(y脿n)鏂规
妯�(bi膩o)婧�(zh菙n)鍝佹績搴︼紙pg/ml锛�
A 800 800 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
B 400 400 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
C 200 200 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
D 100 100 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
E 50 50 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
F 25 25 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
G 0 0 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
H 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
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