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200 ng/ml 锛�4铏熸(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�5铏熸(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
100 ng/ml 锛�3铏熸(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�4铏熸(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
50 ng/ml 锛�2铏熸(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�3铏熸(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
25 ng/ml 锛�1铏熸(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�2铏熸(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
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3锛� 鍔犲叆绋�閲嬪ソ鍚庣殑妯�(bi膩o)婧�(zh菙n)鍝�50ul浜庡弽鎳�(y墨ng)瀛斻�佸姞鍏ュ緟娓ǎ鍝�50ul浜庡弽鎳�(y墨ng)瀛斿収(n猫i)銆傜珛鍗冲姞鍏�50ul鐨勭敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨銆傝搵涓婅啘鏉匡紝杓曡紩鎸暕娣峰嫽锛�37鈩冩韩鑲�1灏忔檪銆�
4锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑诧紝鐢ㄥ惛姘寸礄鎷嶅共銆傞噸寰�(f霉)姝ゆ搷浣�3娆°�傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆°��
5锛� 姣忓瓟鍔犲叆80ul鐨勮Κ鍜岄張閰剁礌-HRP锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�30鍒嗛悩銆�
6锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑诧紝鐢ㄥ惛姘寸礄鎷嶅共銆傞噸寰�(f霉)姝ゆ搷浣�3娆°�傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆°��
7锛� 姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50ul锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�10鍒嗛悩銆傞伩鍏嶅厜鐓с��
8锛� 鍙栧嚭閰舵(bi膩o)鏉匡紝杩呴�熷姞鍏�50ul绲傛娑诧紝鍔犲叆绲傛娑插悗鎳�(y墨ng)绔嬪嵆娓畾绲�(ji茅)鏋溿��
9锛� 鍦�450nm娉㈤暦铏曟脯瀹氬悇瀛旂殑OD鍊笺��寤鸿浣跨敤鐨勫椹楁柟妗�
妯�(bi膩o)婧�(zh菙n)鍝佹績搴︼紙ng/ml锛�
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B 400 400 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
C 200 200 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
D 100 100 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
E 50 50 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
F 25 25 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
G 0 0 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
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