鏈珯瀹㈡埗鏈嶅嫏(w霉)铏�
鐠�(hu谩n)淇濆湪绶氬畼寰�
鐠�(hu谩n)淇濆湪绶氭墜姗�(j墨)鐗�
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
榻婁竴鐢熺墿绉戞妧锛堜笂娴凤級鏈夐檺鍏徃浣滆��
璋锋皑閰革紙glutamic acid锛孏lu锛夊惈閲忚│鍔戠洅
| 璩囨枡椤炲瀷 | png鏂囦欢 | 璩囨枡澶у皬 | 23828 |
| 涓嬭級娆℃暩(sh霉) | 51 | 璩囨枡鍦栫墖 | 銆愰粸(di菐n)鎿婃煡鐪嬨�� |
| 涓� 鍌� 浜� | 榻婁竴鐢熺墿绉戞妧锛堜笂娴凤級鏈夐檺鍏徃 | 闇�瑕佺⿳鍒� | 0 |
| 闂�(gu膩n) 閸� 瑭� | 璋锋皑閰革紙glutamic acid,Glu锛夊惈閲忚│鍔戠洅 | ||
- 銆愯硣鏂欑啊浠嬨��
璋锋皑閰�(glutamic acid锛孏lu)鍚噺娓畾瑭﹀姂鐩�瑾槑鏇稿垎鍏夊厜搴︽硶 50 绠�/48 妯�
姝ｅ紡娓畾鍓嶅嫏(w霉)蹇呭彇2-3鍊�(g猫)闋�(y霉)鏈熷樊鐣拌純澶х殑妯ｆ湰鍋氶爯(y霉)娓畾
娓畾鎰忕京锛�
Gu寤ｆ硾瀛樺湪浜庡嫊(d貌ng)鐗�銆佹鐗�銆佸井鐢熺墿鍜屽煿椁�(y菐ng)绱�(x矛)鑳炰腑锛屼笉鍍呮槸绲勬垚铔嬬櫧璩�(zh矛)鐨�20 绋皑鍩洪吀涔嬩竴锛�
鑰屼笖閫氶亷杞�(zhu菐n)姘ㄥ熀浣滅敤鍙冭垏澶氱ó姘ㄥ熀閰稿悎鎴�锛屾槸鐢熺墿楂斿収(n猫i)涓昏姘ㄥ熀渚嗘簮涔嬩竴銆傛澶�锛孏lu閭勬槸
鍛崇簿鐨勪富瑕佹湁鏁堟垚鍒�锛屽父鐢ㄥ仛椋熷搧娣诲姞鍔戜互鍙婇鏂欑敓鐢�(ch菐n)銆�
娓畾鍘熺悊锛�
鍒╃敤鎻愬彇娑叉彁鍙�锛岀劧鍚庣敤椤壊鍔戦��(j矛n)琛岄’鑹�锛岄’鑹插悗鍦� 570nm 涓嬮��(j矛n)琛屾脯瀹氥��
璋锋皑閰�(glutamic acid锛孏lu)鍚噺娓畾瑭﹀姂鐩�闇�鑷倷鐨勫剙鍣ㄥ拰鐢ㄥ搧锛�
鍙鍒嗗厜鍏夊害瑷�(j矛)銆佽嚭寮忛洟蹇冩(j墨)銆佸彲瑾�(di脿o)寮忕Щ娑插櫒銆�1 mL 鐜荤拑姣旇壊鐨�銆佺爺缂姐�佸啺銆佽捀椁炬按銆�
瑭﹀姂鐨勭祫鎴愬拰閰嶅埗锛�
瑭﹀姂涓�锛氭恫楂�100mL×1 鐡�锛�4鈩冧繚瀛橈紱
瑭﹀姂浜岋細绮夊姂×1 鐡讹紝 4鈩冧繚瀛�锛涜嚚鐢ㄥ墠鍔犲叆 10mL 钂搁ぞ姘�锛屽厖鍒嗘贩鍕绘憾瑙�锛岀敤涓嶅畬鐨勮│鍔�
浠� 4鈩冧繚瀛�銆�
璋锋皑閰告彁鍙栵細
1銆佺窗(x矛)鑿屾垨鍩归(y菐ng)绱�(x矛)鑳炴ǎ鍝侊細鍏堟敹闆嗙窗(x矛)鑿屾垨绱�(x矛)鑳炲埌闆㈠績绠″収(n猫i)锛岄洟蹇冨悗妫勪笂娓�锛涙寜鐓х窗(x矛)鑿屾垨绱�(x矛)鑳�
鏁�(sh霉)閲忥紙104
鍊�(g猫)锛夛細瑭﹀姂涓�楂旂⿳锛坢L锛夌偤 500~1000锛�1 鐨勬瘮渚嬶紙寤鸿 1000 钀窗(x矛)鑿屾垨绱�(x矛)鑳炲姞鍏�
2mL 瑭﹀姂涓�锛� 锛岃秴鑱叉尝鐮寸绱�(x矛)鑿屾垨绱�(x矛)鑳烇紙鍐版荡锛屽姛鐜� 20锛呮垨 200W锛岃秴鑱� 3s锛岄枔闅� 10s锛岄噸
寰�(f霉) 30 娆★級锛�8000g 甯告韩闆㈠績 10min锛屽彇涓婃竻锛岀疆鍐颁笂寰呮脯銆�
2銆佺祫绻旀ǎ鍝侊細鎸夌収绲勭箶璩�(zh矛)閲忥紙g锛� 锛氳│鍔戜竴楂旂⿳(mL)鐐� 1锛�5~10 鐨勬瘮渚嬶紙寤鸿绋卞彇绱� 0.2g绲�
绻旓紝鍔犲叆 2mL瑭﹀姂涓�锛�锛岄��(j矛n)琛屽啺娴村嫽婕�銆�8000g 甯告韩闆㈠績 10min锛屽彇涓婃竻锛岀疆鍐颁笂寰呮脯銆�
3銆佽娓咃紙婕匡級鎴栫窗(x矛)鑳炲煿椁�(y菐ng)娑叉ǎ鍝侊細鎸夌収琛�娓咃紙婕匡級鎴栫窗(x矛)鑳炲煿椁�(y菐ng)娑查珨绌嶏紙mL锛� 锛氳│鍔戜竴楂旂⿳
(mL)鐐� 1锛�5~10 鐨勬瘮渚嬶紙寤鸿鍙� 0.2mL 琛�娓咃紙婕匡級鎴栬�呯窗(x矛)鑳炲煿椁�(y菐ng)娑插姞鍏� 2mL 瑭﹀姂涓�锛� 锛岄��(j矛n)
琛屽啺娴村嫽婕裤��8000g 甯告韩闆㈠績10min锛屽彇涓婃竻锛岀疆鍐颁笂寰呮脯銆�
璋锋皑閰�(glutamic acid锛孏lu)鍚噺娓畾瑭﹀姂鐩�娓畾姝ラ
1銆� 鍒嗗厜鍏夊害瑷�(j矛)闋�(y霉)鐔� 30min浠ヤ笂锛岃(di脿o)绡�(ji茅)娉㈤暦鑷� 570nm锛岃捀椁炬按瑾�(di脿o)闆�銆�
2銆� 鍦ㄦ湁钃� EP绠′腑鍔犲叆涓嬪垪瑭﹀姂锛�
瑭﹀姂鍚嶇ū锛�μL锛� 娓畾绠� 灏嶇収绠�
妯ｆ湰 1000
瑭﹀姂涓� 1000
瑭﹀姂浜� 200 200
娣峰嫽锛�90鈩冩按娴�20min锛堣搵绶婏紝浠ラ槻姝㈡按鍒嗘暎澶憋級 锛屾祦姘村喎鍗�锛屼簬570nm娉㈤暦铏曟瘮鑹�锛屸柍A=A
娓畾绠�-A 灏嶇収绠�銆傚皪鐓х鍙鍋氫竴绠�銆�
娉ㄦ剰浜嬮爡(xi脿ng)锛�
鐐烘彁楂樻娓潏鏁忓害锛屾脯瀹氱鐨勫惛鍏夊�兼噳(y墨ng)灏忎簬 1锛岃嫢澶т簬1 鍓囬渶瑕佸皣涓婃竻娑茬敤瑭﹀姂涓�绋�閲嬭嚦閬�
鐣�(d膩ng)鍊嶆暩(sh霉)鍚庢脯瀹�锛岃▓(j矛)绠楀叕寮忎腑涔樹互鐩告噳(y墨ng)绋�閲嬪�嶆暩(sh霉)銆�
璋锋皑閰稿惈閲忚▓(j矛)绠楋細
1銆佹(bi膩o)婧�(zh菙n)姊濅欢涓嬫脯瀹氬洖姝告柟绋嬬偤 y = 0.0328x- 2.423锛� x鐐鸿胺姘ㄩ吀鍚噺锛�µg/mL锛�锛寉鐐哄惛鍏夊�笺��
2銆佹寜鐓ц娓咃紙婕匡級鎴栬�呯窗(x矛)鑳炲煿椁�(y菐ng)娑查珨绌嶈▓(j矛)绠�
璋锋皑閰稿惈閲�(μg/mL)= [(鈻矨 +2.423)÷0.0328×V1]÷(V3×V1÷V2锛�=304.9×(鈻矨+2.423)
3銆佹寜鐓ц泲鐧芥績搴﹁▓(j矛)绠�
璋锋皑閰稿惈閲�(µg/mg prot)=[(鈻矨 +2.423)÷0.0328×V1]÷(V1×Cpr)=30.49×(鈻矨+2.423) ÷Cpr
4銆佹寜鐓фǎ鏈唱(zh矛)閲忚▓(j矛)绠�
璋锋皑閰稿惈閲�(µg/g楫噸)=[(鈻矨 +2.423)÷0.0328×V1]÷(W ×V1÷V2)=61×(鈻矨+2.423) ÷W
5銆佹寜鐓х窗(x矛)鑿屾垨绱�(x矛)鑳炲瘑搴﹁▓(j矛)绠�
璋锋皑閰稿惈閲�(μg/104
cell)=[(鈻矨 +2.423)÷0.0328×V1]÷(1000×V1÷V2)=0.061×(鈻矨+2.423)
V1锛氬姞鍏ュ弽鎳�(y墨ng)楂旂郴涓ǎ鏈珨绌嶏紝1mL锛沄2锛氬姞鍏ユ彁鍙栨恫楂旂⿳锛�2 mL锛沄3锛氬姞鍏ヨ娓咃紙婕匡級
鎴栫窗(x矛)鑳炲煿椁�(y菐ng)娑查珨绌�锛�0.2 mL锛� Cpr锛氭ǎ鏈泲鐧借唱(zh矛)婵冨害锛宮g/mL锛沇锛氭ǎ鏈唱(zh矛)閲忥紝g锛�1000锛�
绱�(x矛)鑿屾垨绱�(x矛)鑳炵附鏁�(sh霉)锛�1000钀�銆�
娉ㄦ剰锛氳┎瑭﹀姂鐩掑儏閬╃敤浜庣櫦(f膩)閰垫恫鎴栫祫绻斾腑璋锋皑閰稿惈閲忔脯瀹�锛屾娓笅闄愮偤70µg/mL銆�璋锋皑閰�(glutamic acid锛孏lu)鍚噺娓畾瑭﹀姂鐩掗綂涓�鐢熺墿鐝�(xi脿n)璨ㄤ緵鎳�(y墨ng)
2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�
div6鏈�3鏃ワ紝2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆戜簬涓婃捣涓ㄥ湅瀹舵渻灞曚腑[瑭崇窗(x矛)]
涓婁竴绡囷細praher閫�(j矛n)鍙ｅ鏂欐鍥為枼
涓嬩竴绡囷細praher濉戞枡纰熼枼璩囨枡
- 鍑℃湰缍�(w菐ng)娉ㄦ槑"渚嗘簮锛氱挵(hu谩n)淇濆湪绶�"鐨勬墍鏈変綔鍝侊紝鐗堟瑠(qu谩n)鍧囧爆浜庣挵(hu谩n)淇濆湪绶氾紝杞�(zhu菐n)杓夎珛蹇呴爤娉ㄦ槑鐠�(hu谩n)淇濆湪绶�锛宧ttp://www.hezebar.com銆傞仌鍙嶈�呮湰缍�(w菐ng)灏囪拷绌剁浉闂�(gu膩n)娉曞緥璨�(z茅)浠�銆�
- 浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛�(sh霉)鏂囩珷銆佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹�锛屽娑夊強渚垫瑠(qu谩n)銆侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂(d膩n)璨�(z茅)浠�锛屾湰缍�(w菐ng)鏈夋瑠(qu谩n)鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨�(z茅)浠�銆�
- 鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭紝骞朵笉浠ｈ〃鏈恫(w菐ng)璐婂悓鍏惰榛�(di菐n)鎴栬瓑瀵�(sh铆)鍏跺収(n猫i)瀹圭殑鐪熷(sh铆)鎬э紝涓嶆壙鎿�(d膩n)姝ら浣滃搧渚垫瑠(qu谩n)琛岀偤鐨勭洿鎺ヨ铂(z茅)浠诲強閫ｅ付璨�(z茅)浠�銆傚叾浠栧獟楂�銆佺恫(w菐ng)绔欐垨鍊�(g猫)浜哄緸鏈恫(w菐ng)杞�(zhu菐n)杓夋檪(sh铆)锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締婧愶紝骞惰嚜璨�(f霉)鐗堟瑠(qu谩n)绛夋硶寰嬭铂(z茅)浠�銆�
- 濡傛秹鍙婁綔鍝佸収(n猫i)瀹�銆佺増娆�(qu谩n)绛夊晱椤�锛岃珛?ji膩n)璋ч珜閽抣(f膩)琛ㄤ箣鏃ヨ捣涓�鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯�锛屽惁鍓囪鐐烘斁妫勭浉闂�(gu膩n)娆�(qu谩n)鍒┿��
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
寰俊鍏溇铏�
