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脫氧核糖核酸酶Ⅱ(豬脾)資料說明
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- 【資料簡介�
英文名稱:DNase II ;Deoxyribonuclease �(porcine spleen);Deoxyribonucleate 3′-oligonucleotido-hydrolase
其他名稱:去氧細(xì)胞核酸酶;氧核糖核酸-5ˊ-寡核苷酸水解酶;無核糖核酸酶;脫氧核糖核酸去聚合�;脫羥核糖核酸酶(豬胰);除氧細(xì)胞核酸酶(豬胰) ;DNA�
CAS號:9025-64-3
級別:BR
類型:Type IV
活力�2000�6000 units/mg
活力定義:One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25�� [Mg2+] = 0.83 mM
性狀(以下信息僅供參�):白色或類白色凍干粉。essentially salt-free。為雙鏈特異性內(nèi)切核酸酶,能降解雙鏈DNA成為具有5ˊ-磷酸�3ˊ-OH末端的寡脫氧核糖核苷酸。當(dāng)有一價錳離子存在�,亦能降解單鏈DNA。溶于水,微溶于30%乙醇和丙酮。酶反應(yīng):脫氧核糖核�→二核苷酸�5′磷�+寡聚核苷酸�5′磷酸zui適pH 7.8。吸光系�(shù)(E1%280)11.1。激活劑為二價金屬離�,zui大激活可以從�(Mg2+)+�(Ca2+)得到。抑制劑有EDTA和十二烷基硫酸鈉??杉?mmol/L�(Ca2+)
用途:本品僅供科研,不得用于其它用��(以下用途僅供參�)用于分子生物�(xué)研究�
保存�-20�
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