鏈珯瀹㈡埗鏈嶅嫏铏�
鐠�(hu谩n)淇濆湪绶氬畼寰�
鐠�(hu谩n)淇濆湪绶氭墜姗熺増
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
姹熻悐鐢熺墿-涓湅鑰佺墝瑭﹀姂渚涙噳鍟�浣滆��
闆濭ARNL1鍩哄洜Elisa瑭﹀姂鐩掍娇鐢ㄨ鏄庢浉
| 璩囨枡椤炲瀷 | 鏂囦欢 | 璩囨枡澶у皬 | |
| 涓嬭級娆℃暩(sh霉) | 0 | 璩囨枡鍦栫墖 | 銆愰粸鎿婃煡鐪嬨�� |
| 涓� 鍌� 浜� | 涓婃捣姹熻悐 | 闇�瑕佺⿳鍒� | 0 |
| 闂� 閸� 瑭� | 闆濭ARNL1鍩哄洜Elisa瑭﹀姂鐩掍娇鐢ㄨ鏄庢浉 | ||
- 銆愯硣鏂欑啊浠嬨��
鏈│鍔戠洅鍙兘鐢ㄤ簬绉戝鐮旂┒锛屼笉寰楃敤浜庨啱(y墨)瀛歌ê鏂枫��
闆�GARNL1鍩哄洜瀹氶噺妾㈡脯瑭﹀姂鐩掞紙ELISA锛�
浣跨敤瑾槑鏇�
銆愯│鍔戠洅鍚嶇ū銆�
闆�GARNL1鍩哄洜瀹氶噺妾㈡脯瑭﹀姂鐩掞紙ELISA锛�
銆愯│鍔戠洅鐢ㄩ��銆�
瀹氶噺妾㈡脯闆炶娓呫�佽婕垮強鐩搁棞娑查珨妯ｆ湰涓�GARNL1鍩哄洜鐨�鍚噺銆�
銆愭娓�鍘熺悊銆�
鏈│鍔戠洅閲囩敤闆欐姉楂斿叐姝ュぞ蹇冮叾鑱�(li谩n)鍏嶇柅鍚搁檮娉曪紙ELISA锛夈�傚皣妯欐簴鍝併�佸緟娓ǎ鏈姞鍏ュ埌闋愬厛鍖呰闆�GARNL1鍩哄洜鍠厠闅嗘姉楂旈�忔槑閰舵鍖呰鏉夸腑锛�婧偛瓒冲鏅傞枔鍚庯紝娲楁粚闄ゅ幓鏈祼鍚堢殑鎴愬垎锛�鍐嶅姞鍏ラ叾妯欏伐浣滄恫锛�婧偛瓒冲鏅傞枔鍚庯紝娲楁粚闄ゅ幓鏈祼鍚堢殑鎴愬垎銆備緷娆″姞鍏ュ簳鐗�A銆�B锛屽簳鐗╋紙TMB锛夊湪杈ｆ牴閬庢哀鍖栫墿閰讹紙HRP锛夊偓鍖栦笅杞�(zhu菐n)鍖栫偤钘嶈壊鐢�(ch菐n)鐗╋紝鍦ㄩ吀鐨勪綔鐢ㄤ笅璁婃垚榛冭壊锛�椤忚壊鐨勬繁娣鸿垏妯ｅ搧涓�闆�GARNL1鍩哄洜婵冨害鍛堟鐩搁棞锛�450nm娉㈤暦涓嬫脯瀹�OD鍊硷紝鏍规摎(j霉)妯欐簴鍝佸拰妯ｅ搧鐨�OD鍊硷紝瑷堢畻妯ｆ湰涓�闆�GARNL1鍩哄洜鍚噺銆�
銆愯│鍔戠洅绲勬垚銆�
1
閰舵鍖呰鏉�
12瀛�×8姊�
7
椤壊鍔�A娑�
6mL
2
妯欐簴鍝�
0.3mL×6绠�
8
椤壊鍔�B娑�
6mL
3
20鍊嶆績绺礂婊屾恫
25mL
9
绲傛娑�
6mL
4
*
6mL
10
瑾槑鏇�
1浠�
5
鐗规畩绋�閲嬫恫
6mL
11
灏佹澘鑶�
2寮�
6
閰舵瑭﹀姂
6mL
12
瀵嗗皝琚�
1鍊�
鍌欐敞锛�妯欐簴鍝侊紙1铏�→6铏燂級婵冨害渚濇鐐猴細80銆�40銆�20銆�10銆�5銆�2.5 ng/L.
銆�闇�瑕佽�屾湭鎻愪緵鐨勮│鍔戝拰鍣ㄦ潗銆�
1銆�37鈩�鎭掓韩绠�
2銆�妯欐簴瑕�(gu墨)鏍奸叾妯欏剙
3銆�绮惧瘑绉绘恫鍣ㄥ強涓�娆℃�у惛闋�
4銆�钂搁ぞ姘�
5銆�涓�娆℃�ц│绠�
6銆�鍚告按绱�
銆愭搷浣滄椹熴��
1銆佹簴鍌欙細寰炲啺绠卞彇鍑鸿│鍔戠洅锛屽婧京婧钩琛�30鍒嗛悩銆�
2銆侀厤娑诧細鐢ㄨ捀椁炬按灏�20鍊嶆績绺礂婊屾恫绋�閲嬫垚鍘熷�嶇殑娲楁粚娑层��
3銆佸姞妯欐簴鍝佸拰寰呮脯妯ｆ湰锛氬彇瓒冲鏁�(sh霉)閲忕殑閰舵鍖呰鏉匡紝鍥哄畾浜庢鏋朵笂锛�鍒嗗垾瑷疆妯欐簴鍝佸瓟銆佸緟娓ǎ鏈瓟鍜岀┖鐧藉皪鐓у瓟锛岃閷勫悇瀛斾綅缃紝鍦ㄦ婧栧搧瀛斾腑鍔犲叆妯欐簴鍝�50μL锛涘緟娓ǎ鏈瓟涓厛鍔犲叆寰呮脯妯ｆ湰10μL锛屽啀鍔�*40μL锛堝嵆妯ｆ湰绋�閲�5鍊嶏級锛涚┖鐧藉皪鐓у瓟涓嶅姞銆�
4銆佹韩鑲诧細37鈩冩按娴撮崑鎴栨亽婧婧偛30min銆�
5銆佹礂鏉匡細妫勫幓娑查珨锛屽惛姘寸礄涓婃媿骞诧紝姣忓瓟鍔犳豢娲楁粚娑诧紝闈滅疆1min锛岀敥鍘绘礂婊屾恫锛屽惛姘寸礄涓婃媿骞诧紝濡傛閲嶅京娲楁澘4娆★紙涔熷彲鐢ㄦ礂鏉挎鎸夎鏄庢浉鎿嶄綔娲楁澘锛夈��
6銆佸姞閰舵宸ヤ綔娑诧細姣忓瓟鍔犲叆閰舵宸ヤ綔娑�50μL锛岀┖鐧藉皪鐓у瓟涓嶅姞銆�
7銆佹韩鑲诧細37鈩冩按娴撮崑鎴栨亽婧婧偛30min銆�
8銆佹礂鏉匡細妫勫幓娑查珨锛屽惛姘寸礄涓婃媿骞诧紝姣忓瓟鍔犳豢娲楁粚娑诧紝闈滅疆1min锛岀敥鍘绘礂婊屾恫锛屽惛姘寸礄涓婃媿骞诧紝濡傛閲嶅京娲楁澘4娆★紙涔熷彲鐢ㄦ礂鏉挎鎸夎鏄庢浉鎿嶄綔娲楁澘锛夈��
9銆侀’鑹诧細姣忓瓟鍏堝姞鍏ラ’鑹插姂A 娑�50μL锛屽啀鍔犲叆椤壊鍔�B娑� 50μL锛屽钩鏉挎贩鍕诲櫒娣峰嫽30s锛堟垨鐢ㄦ墜杓曡紩闇囪暕娣峰嫽30s锛夛紝37鈩冮伩鍏夐’鑹�15min銆�
10銆佺祩姝細鍙栧嚭閰舵鏉匡紝姣忓瓟鍔犵祩姝㈡恫50μL锛岀祩姝㈠弽鎳夛紙椤忚壊鐢辫棈鑹茬珛杞�(zhu菐n)榛冭壊锛夈��
11銆佹脯瀹氾細浠ョ┖鐧藉瓟瑾�(di脿o)闆讹紝鍦ㄧ祩姝㈠悗15鍒嗛悩鍏�(n猫i)锛岀敤450nm娉㈤暦娓噺鍚勫瓟鐨勫惛鍏夊�硷紙OD鍊硷級銆�
12銆佽▓绠楋細鏍规摎(j霉)妯欐簴鍝佺殑婵冨害鍙婂皪鎳夌殑OD鍊硷紝瑷堢畻鍑烘婧栨洸绶氱殑鐩寸窔鍥炴鏂圭▼锛屽啀鏍规摎(j霉)妯ｆ湰鐨�OD鍊硷紝鍦ㄥ洖姝告柟绋嬩笂瑷堢畻鍑哄皪鎳夌殑妯ｅ搧婵冨害锛屼篃鍙互浣跨敤鍚勭ó鎳夌敤杌熶欢渚嗚▓绠椼�倆ui绲傛績搴︾偤瀵﹂殯娓畾婵冨害涔樹互绋�閲嬪�嶆暩(sh霉)銆�
銆愭ǎ鏈姹傘��
1銆佹ǎ鏈�涓嶈兘鍚枈姘垑锛圢aN3锛�锛屽洜鐐�鐤婃爱閳夛紙NaN3锛夋槸杈ｆ牴閬庢哀鍖栫墿閰讹紙HRP锛夌殑鎶戝埗鍔戙��
2銆�妯欐湰閲囬泦鍚庣洝鏃╅�茶鎻愬彇锛屾彁鍙栨寜鐩搁棞鏂囩嵒閫茶锛屾彁鍙栧悗鎳夌洝蹇�茶瀵﹂銆傝嫢涓嶈兘绔嬪嵆瑭﹂锛屽彲灏囨鏈斁浜�-20鈩冧繚瀛橈紝浣嗘噳閬垮厤鍙嶅京鍑嶈瀺銆�
3銆佹ǎ鏈噳鍏呭垎闆㈠績锛屼笉寰楁湁婧惰鍙婇绮掋��
銆愭敞鎰忎簨闋呫��
1銆佸椹楀毚鏍兼寜鐓ц鏄庢浉鐨勬搷浣滈�茶锛屽椹楃祼鏋滃垽瀹氬繀闋堜互閰舵鍎�璁�鏁�(sh霉)鐐烘簴銆�
2銆�閰舵鍖呰鏉块枊灏佸悗濡傛湭鐢ㄥ畬锛屾噳绔嬪嵆瑁濆叆瀵嗗皝琚嬩腑鍔犲共鐕ュ姂淇濆瓨銆�
3銆佸缓璀版墍鏈夌殑妯欐簴鍝併�佹ǎ鏈拰绌虹櫧灏嶇収閮藉仛闆欎唤妾㈡脯锛屽彇骞冲潎鍊硷紝浠ユ笡灏忓椹楄宸��
4銆佺墷瑷樻ǎ鏈凡缍�(j墨ng)绋�閲�5鍊嶏紝瑷堢畻绲愭灉涔樹互5鎵嶆槸妯ｆ湰瀵﹂殯婵冨害銆�
5銆佹湰瑭﹀姂鐩掑畾閲忚寖鍦嶇偤2.5-80ng/L锛岃秴閬庢鑼冨湇锛岀偤妯欐簴鏇茬窔寤朵几瑷堢畻鎵�寰楋紝涓嶅仛鐐烘簴纰哄畾閲忕祼鏋滐紝璜嬬敤鐗规畩绋�閲嬫恫绋�閲嬪悗娓畾婧栫⒑绲愭灉锛�2.5-80ng/L鑼冨湇鍏�(n猫i)锛夛紝涔樹互绺界█閲嬪�嶆暩(sh霉)鍗崇偤妯ｆ湰zui绲傛績搴︺��
6銆佽嫢椤壊閬庢泛锛屽彲閬╃暥寤堕暦搴曠墿婧偛鏅傞枔銆�
7銆佺偤閬垮厤浜ゅ弶姹℃煋锛屾婧栧搧銆佹ǎ鏈拰绌虹櫧灏嶇収姣忓姞涓�鍊嬪氨瑕佹洿鎻涗竴娆″惛闋紱閰舵宸ヤ綔娑层��*鍜屽簳鐗╃瓑鍏叡绲勫垎锛岃鎳歌噦鍔犳ǎ锛屼笉寰楃鍒板井瀛�锛涗笉寰楅噸寰╀娇鐢�灏佹澘鑶�銆�
8銆佽│鍔戠洅淇濊唱(zh矛)鏈熷収(n猫i)浣跨敤锛屼笉鍚屾壒铏熺殑瑭﹀姂涓嶅緱娣风敤銆�
9銆佸簳鐗�B灏嶅厜鏁忔劅锛岄伩鍏嶉暦鏅傞枔鏆撮湶浜庡厜涓嬨��
銆愭搷浣滅▼搴忕附绲愩��
婧栧倷瑭﹀姂锛屾ǎ鍝佸拰妯欐簴鍝�
瑷堢畻
銆�妾㈡脯鑼冨湇銆�
2.5ng/L - 80ng/L
銆愯(gu墨)鏍笺��
96浜轰唤/鐩�
銆愯帛钘忋��
2-8鈩冿紝閬垮厜闃叉疆淇濆瓨銆�
銆愭湁鏁堟湡銆�
6鍊嬫湀
2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�
div6鏈�3鏃ワ紝2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆戜簬涓婃捣涓ㄥ湅瀹舵渻灞曚腑[瑭崇窗]
涓婁竴绡囷細鎰忓ぇ鍒﹕eko瑷堥噺娉垫妧琛撶壒寰佸強瀹夎灏哄鍦�
涓嬩竴绡囷細RP100鍦ㄧ窔ph瑷堜娇鐢ㄨ鏄庢浉
- 鍑℃湰缍�(w菐ng)娉ㄦ槑"渚嗘簮锛氱挵(hu谩n)淇濆湪绶�"鐨勬墍鏈変綔鍝侊紝鐗堟瑠鍧囧爆浜庣挵(hu谩n)淇濆湪绶氾紝杞�(zhu菐n)杓夎珛蹇呴爤娉ㄦ槑鐠�(hu谩n)淇濆湪绶氾紝http://www.hezebar.com銆傞仌鍙嶈�呮湰缍�(w菐ng)灏囪拷绌剁浉闂滄硶寰嬭铂浠汇��
- 浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛撴枃绔犮�佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹癸紝濡傛秹鍙婁镜娆娿�侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂璨换锛屾湰缍�(w菐ng)鏈夋瑠鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨换銆�
- 鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭紝骞朵笉浠ｈ〃鏈恫(w菐ng)璐婂悓鍏惰榛炴垨璀夊鍏跺収(n猫i)瀹圭殑鐪熷鎬э紝涓嶆壙鎿旀椤炰綔鍝佷镜娆婅鐐虹殑鐩存帴璨换鍙婇�ｅ付璨换銆傚叾浠栧獟楂斻�佺恫(w菐ng)绔欐垨鍊嬩汉寰炴湰缍�(w菐ng)杞�(zhu菐n)杓夋檪锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締婧愶紝骞惰嚜璨犵増娆婄瓑娉曞緥璨换銆�
- 濡傛秹鍙婁綔鍝佸収(n猫i)瀹广�佺増娆婄瓑鍟忛锛岃珛鍦ㄤ綔鍝佺櫦(f膩)琛ㄤ箣鏃ヨ捣涓�鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯伙紝鍚﹀墖瑕栫偤鏀炬鐩搁棞娆婂埄銆�
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
寰俊鍏溇铏�
