澶ч紶瓒呮哀鍖栫墿姝у寲閰讹紙SOD锛塃LISA妾㈡脯(c猫)瑭﹀姂鐩�

2014骞�03鏈�04鏃� 09:48:40浜烘埃锛�176渚嗘簮锛�涓婃捣钄氶渾鐢熺墿绉戞妧鏈夐檺鍏徃

璩囨枡椤炲瀷	doc鏂囦欢	璩囨枡澶у皬	218112
涓嬭級娆℃暩(sh霉)	13	璩囨枡鍦栫墖	銆愰粸(di菐n)鎿婃煡鐪嬨€�
涓� 鍌� 浜�	涓婃捣钄氶渾鐢熺墿绉戞妧鏈夐檺鍏徃	闇€瑕佺⿳鍒�	0
闂�(gu膩n) 閸� 瑭�	澶ч紶瓒呮哀鍖栫墿姝у寲閰�,锛圫OD锛�,ELISA妾㈡脯(c猫)瑭﹀姂鐩�

銆愯硣鏂欑啊(ji菐n)浠嬨€�

涓婃捣閫告櫁鐢熺墿绉戞妧鏈夐檺鍏徃浠ｇ悊涓嶅悓鍝佺墝鍍�(ji脿)鏍兼獢娆＄殑ELISA瑭﹀姂鐩掋€傛姉楂旂敘(ch菐n)鍝佺瓑, 鍝佺ó澶氾紝璩�(zh矛)閲忓ソ锛屽児(ji脿)鏍煎(sh铆)鎯狅紝骞朵笖閭勬彁渚涘厤璨�(f猫i)浠ｆ娓�(c猫)鏈嶅嫏(w霉)銆�
鏈叕鍙哥殑鏇村鐢�(ch菐n)鍝侊紝璜�(q菒ng)榛�(di菐n)鎿婂叕鍙革細http://www.yihanbio.com/

鏈│鍔戠洅鍙兘鐢ㄤ簬绉戝(xu茅)鐮旂┒锛屼笉寰楃敤浜庨啱(y墨)瀛�(xu茅)瑷烘柗

澶ч紶瓒呮哀鍖栫墿姝у寲閰讹紙SOD锛�ELISA妾㈡脯(c猫)瑭﹀姂鐩�

浣跨敤瑾槑鏇�

妾㈡脯(c猫)鍘熺悊

瑭﹀姂鐩掗噰鐢ㄩ洐鎶楅珨涓€姝ュぞ蹇冩硶閰惰伅(li谩n)鍏嶇柅鍚搁檮瑭﹂(y脿n)锛圗LISA锛夈€傚線闋�(y霉)鍏堝寘琚ぇ榧犺秴姘у寲鐗╂鍖栭叾锛圫OD锛夋崟鐛叉姉楂旂殑鍖呰寰瓟涓紝渚濇鍔犲叆妯�(bi膩o)鏈€佹(bi膩o)婧�(zh菙n)鍝併€丠RP妯�(bi膩o)瑷樼殑妾㈡脯(c猫)鎶楅珨锛岀稉(j墨ng)閬庢韩鑲插苟*娲楁粚銆傜敤搴曠墿TMB椤壊锛孴MB鍦ㄩ亷姘у寲鐗╅叾鐨勫偓鍖栦笅杞�(zhu菐n)鍖栨垚钘�(l谩n)鑹诧紝骞跺湪閰哥殑浣滅敤涓嬭綁(zhu菐n)鍖栨垚zui绲傜殑榛冭壊銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑鐨勫ぇ榧犺秴姘у寲鐗╂鍖栭叾锛圫OD锛夊憟姝ｇ浉闂�(gu膩n)銆傜敤閰舵(bi膩o)鍎€鍦�450nm 娉㈤暦(zh菐ng)涓嬫脯(c猫)瀹氬惛鍏�搴︼紙OD 鍊硷級锛岃▓(j矛)绠楁ǎ鍝佹績搴︺€�

妯ｅ搧鏀堕泦銆佽檿鐞嗗強淇濆瓨鏂规硶

1. 琛€娓咃細浣跨敤涓嶅惈鐔卞師鍜屽収(n猫i)姣掔礌鐨勮│绠★紝鎿嶄綔閬庣▼涓伩鍏嶄换浣曠窗(x矛)鑳炲埡婵€锛屾敹闆嗚娑插悗锛�3000杞�(zhu菐n)闆㈠績10鍒嗛悩灏囪娓呭拰绱呯窗(x矛)鑳炶繀閫熷皬蹇冨湴鍒嗛洟銆�

2. 琛€婕匡細EDTA銆佹妾吀楣芥垨鑲濈礌鎶楀嚌銆�3000杞�(zhu菐n)闆㈠績30鍒嗛悩鍙栦笂娓呫€�

3. 绱�(x矛)鑳炰笂娓呮恫锛�3000杞�(zhu菐n)闆㈠績10鍒嗛悩鍘婚櫎椤嗙矑鍜岃仛鍚堢墿銆�

4. 绲勭箶鍕绘伎锛氬皣绲勭箶鍔犲叆閬╅噺鐢熺悊楣芥按鎼楃銆�3000杞�(zhu菐n)闆㈠績10鍒嗛悩鍙栦笂娓呫€�

5. 淇濆瓨锛氬鏋滄ǎ鏈敹闆嗗悗涓嶅強鏅�(sh铆)妾㈡脯(c猫)锛岃珛(q菒ng)鎸変竴娆＄敤閲忓垎瑁濓紝鍑嶅瓨浜�-20鈩冿紝閬垮厤鍙嶅京(f霉)鍑嶈瀺锛屽湪瀹ゆ韩涓嬭В鍑嶅苟纰轰繚妯ｅ搧鍧囧嫽鍦板厖鍒嗚В鍑嶃€�

鑷倷鐗╁搧

閰舵(bi膩o)鍎€锛�450nm锛�
楂樼簿搴﹀姞妯ｅ櫒鍙婃闋細0.5-10uL銆�2-20uL銆�20-200uL銆�200-1000uL
37鈩�鎭掓韩绠�

鎿嶄綔娉ㄦ剰浜嬮爡(xi脿ng)

瑭﹀姂鐩掍繚瀛樺湪2-8鈩冿紝浣跨敤鍓嶅婧钩琛�20鍒嗛悩銆傚緸鍐扮鍙栧嚭鐨勬績绺礂婊屾恫鏈�(hu矛)鏈夌祼(ji茅)鏅讹紝閫欏爆浜庢甯哥従(xi脿n)璞★紝姘存荡鍔犵啽浣跨祼(ji茅)鏅�*婧惰В鍚庡啀浣跨敤銆�
瀵�(sh铆)椹�(y脿n)涓笉鐢ㄧ殑鏉挎鎳�(y墨ng)绔嬪嵆鏀惧洖鑷皝琚嬩腑锛屽瘑灏侊紙浣庢韩骞茬嚗锛変繚瀛樸€�
闋�(y霉)铏曠悊鍚庣殑妯ｆ湰鐒￠渶绋€閲嬶紝鐩存帴鍙�50μL鍔犳ǎ鍗冲彲銆�
鍤�(y谩n)鏍兼寜鐓ц鏄庢浉涓(bi膩o)鏄庣殑鏅�(sh铆)闁撱€佸姞娑查噺鍙婇爢搴忛€�(j矛n)琛屾韩鑲叉搷浣溿€�
鎵€鏈夋恫楂旂祫鍒嗕娇鐢ㄥ墠鍏呭垎鎼栧嫽銆�

瑭﹀姂鐩掔祫鎴�

鍚嶇ū	96瀛旈厤缃�	48瀛旈厤缃�	鍌欐敞
寰瓟閰舵(bi膩o)鏉�	12瀛�×8姊�	12瀛�×4姊�	鐒�
妯�(bi膩o)婧�(zh菙n)鍝侊紙6绠★級	0.5ml/绠�	0.5mL/绠�	鐒�
*	6mL	3mL	鐒�
妾㈡脯(c猫)鎶楅珨-HRP	10mL	5mL	鐒�
20×娲楁粚绶╂矕娑�	25mL	15mL	鎸夎鏄庢浉閫�(j矛n)琛岀█閲�
搴曠墿A	6mL	3mL	鐒�
搴曠墿B	6mL	3mL	鐒�
绲傛娑�	6mL	3mL	鐒�
灏佹澘鑶�	2寮�	2寮�	鐒�
瑾槑鏇�	1浠�	1浠�	鐒�
鑷皝琚�	1鍊�(g猫)	1鍊�(g猫)	鐒�

娉細

1銆佹(bi膩o)婧�(zh菙n)鍝佹績搴︿緷娆＄偤锛�200銆�100銆�50銆�25銆�12.5銆�6.25 U/mL.

2銆佸湪妯ｆ湰鍊艰秴閬庢(bi膩o)婧�(zh菙n)鍝亃ui楂樻績搴︾殑鎯呮硜涓嬶紝鍙敤*閬╃暥(d膩ng)绋€閲嬫ǎ鏈€�

瑭﹀姂鐨勬簴(zh菙n)鍌�

20×娲楁粚绶╂矕娑茬殑绋€閲嬶細钂搁ぞ姘存寜1锛�20绋€閲嬶紝鍗�1浠界殑20×娲楁粚绶╂矕娑插姞19浠界殑钂搁ぞ姘淬€�

娲楁澘鏂规硶

鎵嬪伐娲楁澘锛氱敥鐩″瓟鍏�(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛岄潨缃�1min鍚庣敥鐩″瓟鍏�(n猫i)娑查珨锛屽湪鍚告按绱欎笂鎷嶅共锛屽姝ゆ礂鏉�5娆°€�
鑷嫊(d貌ng)娲楁澘姗�(j墨)锛氭瘡瀛旀敞鍏ユ礂娑�350μL锛屾蹈娉�1min锛屾礂鏉�5娆°€�

鎿嶄綔姝ラ

寰炲婧钩琛�20min鍚庣殑閶佺當琚嬩腑鍙栧嚭鎵€闇€鏉挎锛屽墿浣欐澘姊濈敤鑷皝琚嬪瘑灏佹斁鍥�4鈩冦€�
瑷�(sh猫)缃(bi膩o)婧�(zh菙n)鍝佸瓟銆佹ǎ鏈瓟鍜岀┖鐧藉瓟锛�绌虹櫧瀛斾粈涔堥兘涓嶅姞锛涙(bi膩o)婧�(zh菙n)鍝佸瓟鍚勫姞涓嶅悓婵冨害鐨勬(bi膩o)婧�(zh菙n)鍝�50μL锛�
寰呮脯(c猫)妯ｆ湰瀛斿悇鍔犲緟娓�(c猫)妯ｆ湰50μL锛�
闅ㄥ悗妯�(bi膩o)婧�(zh菙n)鍝佸瓟鍜屾ǎ鏈瓟涓�锛堢┖鐧藉瓟涓嶅姞锛�鍔犲叆杈ｆ牴閬庢哀鍖栫墿閰讹紙HRP锛夋(bi膩o)瑷樼殑妾㈡脯(c猫)鎶楅珨100μL锛岋紝鐢ㄥ皝鏉胯啘灏佷綇鍙嶆噳(y墨ng)瀛旓紝37鈩冩按娴撮崑鎴栨亽婧婧偛60min銆�
妫勫幓娑查珨锛屽惛姘寸礄涓婃媿骞诧紝姣忓瓟鍔犳豢娲楁粚娑诧紝闈滅疆1min锛岀敥鍘绘礂婊屾恫锛屽惛姘寸礄涓婃媿骞诧紝濡傛閲嶅京(f霉)娲楁澘5娆★紙涔熷彲鐢ㄦ礂鏉挎(j墨)娲楁澘锛夈€�
鎵€鏈夊瓟鍔犲叆搴曠墿A銆丅鍚�50μL锛�37鈩冮伩鍏夊鑲�15min銆�
鎵€鏈夊瓟鍔犲叆绲傛娑�50μL锛�15min鍏�(n猫i)锛屽湪450nm娉㈤暦(zh菐ng)铏曟脯(c猫)瀹氬悇瀛旂殑OD鍊笺€�

绲�(ji茅)鏋滃垽鏂�

绻埗妯�(bi膩o)婧�(zh菙n)鏇茬窔锛氬湪Excel宸ヤ綔琛ㄤ腑锛屼互妯�(bi膩o)婧�(zh菙n)鍝佹績搴︿綔姗潗妯�(bi膩o)锛屽皪(du矛)鎳�(y墨ng)OD鍊间綔绺卞潗妯�(bi膩o)锛岀躬鍒跺嚭妯�(bi膩o)婧�(zh菙n)鍝佺窔鎬у洖姝告洸绶氾紝鎸夋洸绶氭柟绋嬭▓(j矛)绠楀悇妯ｆ湰婵冨害鍊笺€�

瑭﹀姂鐩掓€ц兘

1. 婧�(zh菙n)纰烘€э細妯�(bi膩o)婧�(zh菙n)鍝佺窔鎬у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊硷紝澶т簬绛変簬0.9900銆�

2. 闈堟晱搴︼細zui浣庢娓�(c猫)婵冨害灏忎簬1.0 U/mL銆�

3. 鐗圭暟鎬э細涓嶈垏鍏跺畠鍙憾鎬х祼(ji茅)妲�(g貌u)椤炰技鐗╀氦鍙夊弽鎳�(y墨ng)銆�

4. 閲嶅京(f霉)鎬э細鏉垮収(n猫i)銆佹澘闁撹畩鐣扮郴鏁�(sh霉)鍧囧皬浜�15%銆�

5. 璨棌锛�2-8鈩冿紝閬垮厜闃叉疆淇濆瓨銆�

6. 鏈夋晥鏈燂細6鍊�(g猫)鏈�

鍏嶈铂(z茅)鑱叉槑

1. 瑭﹀姂鐩掑儏渚涚爺绌朵娇鐢紝涓嶅緱鐢ㄤ簬鑷ㄥ簥瀵�(sh铆)椹�(y脿n)鎴栦汉楂斿(sh铆)椹�(y脿n)锛屽惁鍓囨墍鐢�(ch菐n)鐢熺殑涓€鍒囧悗鏋滐紝鐢卞(sh铆)椹�(y脿n)鑰呮壙鎿�(d膩n)锛屾湰鍏徃姒備笉璨�(f霉)璨�(z茅)銆�

2. 鍤�(y谩n)鏍兼寜鐓ц鏄庢浉鎿嶄綔锛屽(sh铆)椹�(y脿n)鑰呴仌鍙嶈鏄庢浉鎿嶄綔锛屽悗鏋滅敱瀵�(sh铆)椹�(y脿n)鑰呮壙鎿�(d膩n)銆�

涓婃捣钄氶渾鐢熺墿绉戞妧鏈夐檺鍏徃浣滆€�

閫�(j矛n)鍏ュ睍鑷�(t谩i)鍦ㄧ窔鐣欒█

2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡€(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�

div6鏈�3鏃ワ紝2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡€(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆戜簬涓婃捣涓ㄥ湅(gu贸)瀹舵渻(hu矛)灞曚腑[瑭崇窗(x矛)]

涓婁竴绡囷細楂樼垚娌栨福姘撮亷婵惧櫒浣跨敤瑾槑

涓嬩竴绡囷細寰挵(hu谩n)姘存梺婵惧櫒鎿嶄綔瑕�(gu墨)绋嬭鏄�

鐗堟瑠(qu谩n)鑸囧厤璨�(z茅)鑱叉槑

鍑℃湰缍�(w菐ng)娉ㄦ槑"渚嗘簮锛氱挵(hu谩n)淇濆湪绶�"鐨勬墍鏈変綔鍝侊紝鐗堟瑠(qu谩n)鍧囧爆浜庣挵(hu谩n)淇濆湪绶氾紝杞�(zhu菐n)杓夎珛(q菒ng)蹇呴爤娉ㄦ槑鐠�(hu谩n)淇濆湪绶氾紝http://www.hezebar.com銆傞仌鍙嶈€呮湰缍�(w菐ng)灏囪拷绌剁浉闂�(gu膩n)娉曞緥璨�(z茅)浠汇€�
浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛�(sh霉)鏂囩珷銆佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹癸紝濡傛秹鍙婁镜娆�(qu谩n)銆侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂(d膩n)璨�(z茅)浠伙紝鏈恫(w菐ng)鏈夋瑠(qu谩n)鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨�(z茅)浠汇€�
鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭紝骞朵笉浠ｈ〃鏈恫(w菐ng)璐婂悓鍏惰榛�(di菐n)鎴栬瓑瀵�(sh铆)鍏跺収(n猫i)瀹圭殑鐪熷(sh铆)鎬э紝涓嶆壙鎿�(d膩n)姝ら浣滃搧渚垫瑠(qu谩n)琛岀偤鐨勭洿鎺ヨ铂(z茅)浠诲強閫ｅ付璨�(z茅)浠汇€傚叾浠栧獟楂斻€佺恫(w菐ng)绔欐垨鍊�(g猫)浜哄緸鏈恫(w菐ng)杞�(zhu菐n)杓夋檪(sh铆)锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締婧愶紝骞惰嚜璨�(f霉)鐗堟瑠(qu谩n)绛夋硶寰嬭铂(z茅)浠汇€�
濡傛秹鍙婁綔鍝佸収(n猫i)瀹广€佺増娆�(qu谩n)绛夊晱椤岋紝璜�(q菒ng)?ji膩n)璋ч珜閽抣(f膩)琛ㄤ箣鏃ヨ捣涓€鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯伙紝鍚﹀墖瑕栫偤鏀炬鐩搁棞(gu膩n)娆�(qu谩n)鍒┿€�

九一国产精品_日本免费不卡一区二区_在线a毛片_久久在草_国产一区二区影视_亚洲国产美女视频

澶ч紶瓒呮哀鍖栫墿姝у寲閰讹紙SOD锛塃LISA妾㈡脯(c猫)瑭﹀姂鐩�

2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡€(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�

鐗堟瑠(qu谩n)鑸囧厤璨�(z茅)鑱叉槑