鏈珯瀹㈡埗鏈嶅嫏(w霉)铏�
鐠�(hu谩n)淇濆湪绶氬畼寰�
鐠�(hu谩n)淇濆湪绶氭墜姗�(j墨)鐗�
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
涓婃捣閫氳敋鐢熺墿绉戞妧鏈夐檺鍏徃浣滆��
96T浜虹硸鍘熺７閰稿寲閰跺悓宸ラ叾BBELISA 妾㈡脯瑭﹀姂鐩掍娇鐢ㄧ殑瀵�(sh铆)椹�(y脿n)鏂规
| 璩囨枡椤炲瀷 | rar鏂囦欢 | 璩囨枡澶у皬 | 109874 |
| 涓嬭級娆℃暩(sh霉) | 86 | 璩囨枡鍦栫墖 | 銆愰粸(di菐n)鎿婃煡鐪嬨�� |
| 涓� 鍌� 浜� | 涓婃捣閫氳敋鐢熺墿绉戞妧鏈夐檺鍏徃 | 闇�瑕佺⿳鍒� | 0 |
| 闂�(gu膩n) 閸� 瑭� | 浜虹硸鍘熺７閰稿寲閰跺悓宸ラ叾BBELISA 妾㈡脯瑭﹀姂鐩� | ||
- 銆愯硣鏂欑啊浠嬨��
浜虹硸鍘熺７閰稿寲閰跺悓宸ラ叾BBELISA 妾㈡脯瑭﹀姂鐩�
鏈│鍔戝儏渚涚爺绌朵娇鐢� 妯�(bi膩o)鏈細琛�娓呮垨琛�婕�涓婃捣淇＄劧瀵�(sh铆)妤�(y猫)灏堟キ(y猫)渚涙噳(y墨ng)钁¤仛绯栧嚌鑶燞L-20,鐧戒粙绱爀lisa瑭﹀姂鐩�,鑵槫澹炴鍥犲瓙elisa瑭﹀姂鐩�,浜洪鐧戒粙鎶楀師B27,绱�(x矛)鑳炵敓闀峰洜瀛愯│鍔戠洅锛屾彁渚涘厤璨�(f猫i)浠ｆ脯鏈嶅嫏(w霉)锛屼繚璀夊(sh铆)椹�(y脿n)绲�(ji茅)鏋滃瑙�鐪熷(sh铆)鎬с�傛垜鍏徃灏嶈│鍔戠洅璩�(zh矛)閲�100%淇濊瓑锛岃嫢瀛樺湪璩�(zh矛)閲忓晱椤岋紝鎴戝�戠劇姊濅欢鍖呴��鎻�
瑭﹂(y脿n)鍘熺悊锛�
銆�銆�銆�銆�GP-BB瑭﹀姂鐩掓槸鍥虹浉澶惧績娉曢叾鑱�(li谩n)鍏嶇柅鍚搁檮瀵�(sh铆)椹�(y脿n)锛圗LISA锛�.宸茬煡GP-BB婵冨害鐨勬(bi膩o)婧�(zh菙n)鍝併�佹湭鐭ユ績搴︾殑妯ｅ搧鍔犲叆寰瓟閰舵(bi膩o)鏉垮収(n猫i)閫�(j矛n)琛屾娓�傚厛灏嘒P-BB鍜岀敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨鍚屾檪婧偛銆傛礂婊屽悗锛屽姞鍏ヨΚ鍜岀礌妯�(bi膩o)瑷橀亷鐨凥RP銆傚啀缍�(j墨ng)閬庢韩鑲插拰娲楁粚锛屽幓闄ゆ湭绲�(ji茅)鍚堢殑閰剁祼(ji茅)鍚堢墿锛岀劧鍚庡姞鍏ュ簳鐗〢銆丅锛屽拰閰剁祼(ji茅)鍚堢墿鍚屾檪浣滅敤銆傜敘(ch菐n)鐢熼鑹层�傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑GP-BB鐨勬績搴﹀憟姣斾緥闂�(gu膩n)绯汇��
銆�
瀹夊叏鎬�
閬垮厤鐩存帴鎺ヨЦ绲傛娑插拰搴曠墿A銆丅銆備竴鏃︽帴瑙稿埌閫欎簺娑查珨锛岃珛鐩″揩鐢ㄦ按娌栨礂銆�
瀵�(sh铆)椹�(y脿n)涓笉瑕佸悆鍠濄�佹娊鐓欐垨浣跨敤鍖栧鍝併��
涓嶈鐢ㄥ槾鍚稿彇瑭﹀姂鐩掗噷鐨勪换浣曟垚浠姐��浜虹硸鍘熺７閰稿寲閰跺悓宸ラ叾BBELISA 妾㈡脯瑭﹀姂鐩�
鎿嶄綔娉ㄦ剰浜嬮爡
瑭﹀姂鎳�(y墨ng)鎸夋(bi膩o)绨借鏄庢浉鍎插瓨锛屼娇鐢ㄥ墠鎭㈠京(f霉)鍒板婧�傜█绋�閬庡悗鐨勬(bi膩o)婧�(zh菙n)鍝佹噳(y墨ng)涓熸锛屼笉鍙繚瀛樸��
瀵�(sh铆)椹�(y脿n)涓笉鐢ㄧ殑鏉挎鎳�(y墨ng)绔嬪嵆鏀惧洖鍖呰琚嬩腑锛屽瘑灏佷繚瀛橈紝浠ュ厤璁婅唱(zh矛)銆�
涓嶇敤鐨勫叾瀹冭│鍔戞噳(y墨ng)鍖呰濂芥垨钃嬪ソ銆備笉鍚屾壒铏熺殑瑭﹀姂涓嶈娣风敤銆備繚璩�(zh矛)鍓嶄娇鐢ㄣ��
浣跨敤涓�娆℃�х殑鍚搁牠浠ュ厤浜ゅ弶姹℃煋锛屽惛鍙栫祩姝㈡恫鍜屽簳鐗〢銆丅娑叉檪锛岄伩鍏嶄娇鐢ㄥ付閲戝爆閮ㄥ垎鐨勫姞妯ｅ櫒銆�
浣跨敤骞插噲鐨勫鏂欏鍣ㄩ厤缃礂婊屾恫銆備娇鐢ㄥ墠鍏呭垎娣峰嫽瑭﹀姂鐩掗噷鐨勫悇绋垚浠藉強妯ｅ搧銆�
娲楁粚閰舵(bi膩o)鏉挎檪鎳�(y墨ng)鍏呭垎鎷嶅共锛屼笉瑕佸皣鍚告按绱欑洿鎺ユ斁鍏ラ叾妯�(bi膩o)鍙嶆噳(y墨ng)瀛斾腑鍚告按銆�
搴曠墿A鎳�(y墨ng)鎻櫦(f膩)锛岄伩鍏嶉暦鏅傞枔鎵撻枊钃嬪瓙銆傚簳鐗〣灏嶅厜鏁忔劅锛岄伩鍏嶉暦鏅傞枔鏆撮湶浜庡厜涓嬨�傞伩鍏嶇敤鎵嬫帴瑙革紝鏈夋瘨銆傚(sh铆)椹�(y脿n)瀹屾垚鍚庢噳(y墨ng)绔嬪嵆璁�鍙朞D鍊笺��
鍔犲叆瑭﹀姂鐨勯爢搴忔噳(y墨ng)涓�鑷达紝浠ヤ繚璀夋墍鏈夊弽鎳�(y墨ng)鏉垮瓟婧偛鐨勬檪闁撲竴妯ｃ��
鎸夌収瑾槑鏇镐腑妯�(bi膩o)鏄庣殑鏅傞枔銆佸姞娑茬殑閲忓強闋嗗簭閫�(j矛n)琛屾韩鑲叉搷浣溿��妯ｅ搧鏀堕泦銆佽檿鐞嗗強淇濆瓨鏂规硶
琛�娓�-----鎿嶄綔閬庣▼涓伩鍏嶄换浣曠窗(x矛)鑳炲埡婵�銆備娇鐢ㄤ笉鍚啽鍘熷拰鍏�(n猫i)姣掔礌鐨勮│绠°�傛敹闆嗚娑插悗锛�1000×g闆㈠績10鍒嗛悩灏囪娓呭拰绱呯窗(x矛)鑳炶繀閫熷皬蹇冨湴鍒嗛洟銆�
琛�婕�-----EDTA銆佹妾吀楣姐�佽倽绱犺婕垮彲鐢ㄤ簬妾㈡脯銆�1000×g闆㈠績30鍒嗛悩鍘婚櫎椤嗙矑銆�
绱�(x矛)鑳炰笂娓呮恫---1000×g闆㈠績10鍒嗛悩鍘婚櫎椤嗙矑鍜岃仛鍚堢墿銆�
淇濆瓨------濡傛灉妯ｅ搧涓嶇珛鍗充娇鐢紝鎳�(y墨ng)灏囧叾鍒嗘垚灏忛儴鍒�-70 鈩冧繚瀛橈紝閬垮厤鍙嶅京(f霉)鍐峰噸銆傜洝鍙兘鐨勪笉瑕佷娇鐢ㄦ憾琛�鎴栭珮琛�鑴傝銆傚鏋滆娓呬腑澶ч噺椤嗙矑锛屾娓墠鍏堥洟蹇冩垨閬庢烤銆備笉瑕佸湪37鈩冩垨鏇撮珮鐨勬韩搴﹀姞鐔辫В鍑嶃�傛噳(y墨ng)鍦ㄥ婧笅瑙ｅ噸骞剁⒑淇濇ǎ鍝佸潎鍕诲湴鍏呭垎瑙ｅ噸銆�浜虹硸鍘熺７閰稿寲閰跺悓宸ラ叾BBELISA 妾㈡脯瑭﹀姂鐩�
鎿嶄綔姝ラ
浣跨敤鍓嶏紝灏囨墍鏈夎│鍔戝厖鍒嗘贩鍕汇�備笉瑕佷娇娑查珨鐢�(ch菐n)鐢熷ぇ閲忕殑娉℃搏锛屼互鍏嶅姞妯ｆ檪鍔犲叆澶ч噺鐨勬埃娉★紝鐢�(ch菐n)鐢熷姞妯ｄ笂鐨勮宸��
鏍规摎(j霉)寰呮脯妯ｅ搧鏁�(sh霉)閲忓姞涓婃(bi膩o)婧�(zh菙n)鍝佺殑鏁�(sh霉)閲忔焙瀹氭墍闇�鐨勬澘姊濇暩(sh霉)銆傛瘡鍊嬫(bi膩o)婧�(zh菙n)鍝佸拰绌虹櫧瀛斿缓璀板仛寰�(f霉)瀛斻�傛瘡鍊嬫ǎ鍝佹牴鎿�(j霉)鑷繁鐨勬暩(sh霉)閲忎締瀹氾紝鑳戒娇鐢ㄥ京(f霉)瀛旂殑鐩￠噺鍋氬京(f霉)瀛斻��
鍔犲叆绋�閲嬪ソ鍚庣殑妯�(bi膩o)婧�(zh菙n)鍝�50ul浜庡弽鎳�(y墨ng)瀛斻�佸姞鍏ュ緟娓ǎ鍝�50ul浜庡弽鎳�(y墨ng)瀛斿収(n猫i)銆傜珛鍗冲姞鍏�50ul鐨勭敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨銆傝搵涓婅啘鏉匡紝杓曡紩鎸暕娣峰嫽锛�37鈩冩韩鑲�1灏忔檪銆�
鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑诧紝鐢ㄥ惛姘寸礄鎷嶅共銆傞噸寰�(f霉)姝ゆ搷浣�3娆°�傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆°��
姣忓瓟鍔犲叆80ul鐨勮Κ鍜岄張閰剁礌-HRP锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�30鍒嗛悩銆�
鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑诧紝鐢ㄥ惛姘寸礄鎷嶅共銆傞噸寰�(f霉)姝ゆ搷浣�3娆°�傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆°��
姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50ul锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�10鍒嗛悩銆傞伩鍏嶅厜鐓с��
鍙栧嚭閰舵(bi膩o)鏉匡紝杩呴�熷姞鍏�50ul绲傛娑诧紝鍔犲叆绲傛娑插悗鎳�(y墨ng)绔嬪嵆娓畾绲�(ji茅)鏋溿��
鍦�450nm娉㈤暦铏曟脯瀹氬悇瀛旂殑OD鍊笺��6铏熸(bi膩o)婧�(zh菙n)鍝佷互涓婄殑绲�(ji茅)鏋滅偤闈炵窔鎬х殑锛屾牴鎿�(j霉)姝ゆ(bi膩o)婧�(zh菙n)鏇茬窔鐒℃硶寰楀埌鐨勭祼(ji茅)鏋溿��
瑭﹀姂鐩掓�ц兘
1. 闈堟晱搴︼細zui灏忕殑妾㈡脯婵冨害灏忎簬1铏熸(bi膩o)婧�(zh菙n)鍝併�傜█閲嬪害鐨勭窔鎬с�傛ǎ鍝佺窔鎬у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊肩偤0.990銆�
2. 鐗圭暟鎬э細涓嶈垏鍏跺畠绱�(x矛)鑳炲洜瀛愬弽鎳�(y墨ng)銆�
3. 閲嶅京(f霉)鎬э細鏉垮収(n猫i)銆佹澘闁撹畩鐣扮郴鏁�(sh霉)鍧囧皬浜�10%銆�浜虹硸鍘熺７閰稿寲閰跺悓宸ラ叾BBELISA 妾㈡脯瑭﹀姂鐩�
绲�(ji茅) 鏋� 鍒� 鏂� 鑸� 鍒� 鏋�
1銆佸剙鍣ㄥ�硷細浜庢尝闀�450nm鐨勯叾妯�(bi膩o)鍎�涓婅畝鍙栧悇瀛旂殑OD鍊�2銆佷互鍚稿厜搴D鍊肩偤绺卞潗妯�(bi膩o)锛圷锛夛紝鐩告噳(y墨ng)鐨凣P-BB妯�(bi膩o)婧�(zh菙n)鍝佹績搴︾偤姗潗妯�(bi膩o)锛圶锛夛紝鍋氬緱鐩告噳(y墨ng)鐨勬洸绶氾紝妯ｅ搧鐨凣P-BB鍚噺鍙牴鎿�(j霉)鍏禣D鍊肩敱妯�(bi膩o)婧�(zh菙n)鏇茬窔鎻涚畻鍑虹浉鎳�(y墨ng)鐨勬績搴︺��
3銆佹娓�艰寖鍦嶏細0-800pg/ml
4銆佹晱鎰熷害锛� 1.0 pg/ml
Goat anti-rat IgG/APC 鐔掑厜绱燗PC妯�(bi膩o)瑷樼緤鎶楀ぇ榧營gG 0.1ml
Goat anti-rat IgM/APC 鐔掑厜绱燗PC妯�(bi膩o)瑷樼緤鎶楀ぇ榧營gM 0.1ml
Goat anti-Guinea IgG/APC 鐔掑厜绱燗PC缇婃姉璞氶紶IgG 0.1ml
NDRG2锛圢-myc downstream regulated gene 2锛� 鎶戠檶鍩哄洜NDRG2鎶楅珨 0.2ml
NDRG4锛圢-myc downstream regulated gene 4锛� 鎶戠檶鍩哄洜NDRG4鎶楅珨 0.2ml
NCX1锛圢a+/Ca2+ exchanger1锛� 閳夐垼浜ゆ彌铔嬬櫧1鎶楅珨 0.2ml
NCX2/SLC8A2锛坈arrier family 8 锛坰odium/calcium exchanger锛�, member 2锛� 閳夐垼浜ゆ彌铔嬬櫧2鎶楅珨 0.2ml
Nebulin 浼磋倢鍕曡泲鐧芥姉楂� 0.2ml
NCX3锛圢a+/Ca2+ exchanger3锛� 閳夐垼浜ゆ彌铔嬬櫧3鎶楅珨 0.2ml
Mycoplasma Gallisepticum 鎶楅洖姣掓敮鍘熼珨MG鎶楅珨 0.2ml
Nephrin 鍘绘哀鑵庝笂鑵虹礌鎶楅珨 0.1ml
Nephrin 鍘绘哀鑵庝笂鑵虹礌鎶楅珨 0.2ml
Rabbit anti-MK IgG/PE 鐔掑厜绱燩E妯�(bi膩o)瑷樺厰鎶楃尨IgG 0.1ml
Rabbit anti-MK IgM/PE 鐔掑厜绱燩E妯�(bi膩o)瑷樺厰鎶楃尨IgM 0.1ml
Rabbit anti-pig IgG/PE 鐔掑厜绱燩E妯�(bi膩o)瑷樺厰鎶楄爆IgG 0.1ml
Rabbit anti-Pig IgM/PE 鐔掑厜绱燩E妯�(bi膩o)瑷樺厰鎶楄爆IgM 0.1ml
Nestin锛坔uman锛� 宸㈣泲鐧�/绁炵稉(j墨ng)涓婄毊骞茬窗(x矛)鑳炶泲鐧芥姉楂旓紙浜猴級 0.1ml
鑻ラ渶瑕佷簡瑙ｈ│鍔戠洅鐨勮┏绱�(x矛)淇℃伅锛岃珛锛氾細璎�
锛氾紝
2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�
div6鏈�3鏃ワ紝2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆戜簬涓婃捣涓ㄥ湅瀹舵渻灞曚腑[瑭崇窗(x矛)]
涓婁竴绡囷細鐔卞姏绠＄恫(w菐ng)瑁�(b菙)鍙ｇ敤闆荤啽鐔斿鐔辩府甯�
涓嬩竴绡囷細绠￠亾宸ョ▼鐢ㄩ浕鐔辩啍濂� 鐔辩啍濂�
- 鍑℃湰缍�(w菐ng)娉ㄦ槑"渚嗘簮锛氱挵(hu谩n)淇濆湪绶�"鐨勬墍鏈変綔鍝侊紝鐗堟瑠(qu谩n)鍧囧爆浜庣挵(hu谩n)淇濆湪绶氾紝杞�(zhu菐n)杓夎珛蹇呴爤娉ㄦ槑鐠�(hu谩n)淇濆湪绶氾紝http://www.hezebar.com銆傞仌鍙嶈�呮湰缍�(w菐ng)灏囪拷绌剁浉闂�(gu膩n)娉曞緥璨�(z茅)浠汇��
- 浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛�(sh霉)鏂囩珷銆佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹癸紝濡傛秹鍙婁镜娆�(qu谩n)銆侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂(d膩n)璨�(z茅)浠伙紝鏈恫(w菐ng)鏈夋瑠(qu谩n)鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨�(z茅)浠汇��
- 鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭紝骞朵笉浠ｈ〃鏈恫(w菐ng)璐婂悓鍏惰榛�(di菐n)鎴栬瓑瀵�(sh铆)鍏跺収(n猫i)瀹圭殑鐪熷(sh铆)鎬э紝涓嶆壙鎿�(d膩n)姝ら浣滃搧渚垫瑠(qu谩n)琛岀偤鐨勭洿鎺ヨ铂(z茅)浠诲強閫ｅ付璨�(z茅)浠汇�傚叾浠栧獟楂斻�佺恫(w菐ng)绔欐垨鍊嬩汉寰炴湰缍�(w菐ng)杞�(zhu菐n)杓夋檪锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締婧愶紝骞惰嚜璨�(f霉)鐗堟瑠(qu谩n)绛夋硶寰嬭铂(z茅)浠汇��
- 濡傛秹鍙婁綔鍝佸収(n猫i)瀹广�佺増娆�(qu谩n)绛夊晱椤岋紝璜嬪湪浣滃搧鐧�(f膩)琛ㄤ箣鏃ヨ捣涓�鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯伙紝鍚﹀墖瑕栫偤鏀炬鐩搁棞(gu膩n)娆�(qu谩n)鍒┿��
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
寰俊鍏溇铏�
