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Dopamine瑭﹀姂鐩掓槸鍥虹浉澶惧績娉曢叾鑱�(li谩n)鍏嶇柅鍚搁檮瀵﹂锛圗LISA锛�.宸茬煡Dopamine婵冨害鐨勬婧栧搧銆佹湭鐭ユ績搴︾殑妯ｅ搧鍔犲叆寰瓟閰舵鏉垮収(n猫i)閫茶妾㈡脯銆傚厛灏嘍opamine鍜岀敓鐗╃礌妯欒鐨勬姉楂斿悓鏅傛韩鑲层�傛礂婊屽悗锛屽姞鍏ヨΚ鍜岀礌妯欒閬庣殑HRP銆傚啀缍�(j墨ng)閬庢韩鑲插拰娲楁粚锛屽幓闄ゆ湭绲�(ji茅)鍚堢殑閰剁祼(ji茅)鍚堢墿锛岀劧鍚庡姞鍏ュ簳鐗〢銆丅锛屽拰閰剁祼(ji茅)鍚堢墿鍚屾檪浣滅敤銆傜敘(ch菐n)鐢熼鑹层�傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑Dopamine鐨勬績搴﹀憟姣斾緥闂滅郴銆�鑷倷鏉愭枡
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8锛� 鍔犲叆瑭﹀姂鐨勯爢搴忔噳涓�鑷达紝浠ヤ繚璀夋墍鏈夊弽鎳夋澘瀛旀韩鑲茬殑鏅傞枔涓�妯ｃ��
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妯ｅ搧鏀堕泦銆佽檿鐞嗗強淇濆瓨鏂规硶
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3銆� 绱拌優涓婃竻娑�---1000×g闆㈠績10鍒嗛悩鍘婚櫎椤嗙矑鍜岃仛鍚堢墿銆�
4銆� 绲勭箶鍕绘伎-----灏囩祫绻斿姞鍏ラ仼閲忕敓鐞嗛菇姘存悧纰庛��1000×g闆㈠績10鍒嗛悩锛屽彇涓婃竻娑�
5銆� 淇濆瓨------濡傛灉妯ｅ搧涓嶇珛鍗充娇鐢紝鎳夊皣鍏跺垎鎴愬皬閮ㄥ垎-70 鈩冧繚瀛橈紝閬垮厤鍙嶅京鍐峰噸銆傜洝鍙兘鐨勪笉瑕佷娇鐢ㄦ憾琛�鎴栭珮琛�鑴傝銆傚鏋滆娓呬腑澶ч噺椤嗙矑锛屾娓墠鍏堥洟蹇冩垨閬庢烤銆備笉瑕佸湪37鈩冩垨鏇撮珮鐨勬韩搴﹀姞鐔辫В鍑嶃�傛噳鍦ㄥ婧笅瑙ｅ噸骞剁⒑淇濇ǎ鍝佸潎鍕诲湴鍏呭垎瑙ｅ噸銆�鎿嶄綔姝ラ
1锛� 浣跨敤鍓嶏紝灏囨墍鏈夎│鍔戝厖鍒嗘贩鍕汇�備笉瑕佷娇娑查珨鐢�(ch菐n)鐢熷ぇ閲忕殑娉℃搏锛屼互鍏嶅姞妯ｆ檪鍔犲叆澶ч噺鐨勬埃娉★紝鐢�(ch菐n)鐢熷姞妯ｄ笂鐨勮宸��
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3锛� 鍔犲叆绋�閲嬪ソ鍚庣殑妯欐簴鍝�50ul浜庡弽鎳夊瓟銆佸姞鍏ュ緟娓ǎ鍝�50ul浜庡弽鎳夊瓟鍏�(n猫i)銆傜珛鍗冲姞鍏�50ul鐨勭敓鐗╃礌妯欒鐨勬姉楂斻�傝搵涓婅啘鏉匡紝杓曡紩鎸暕娣峰嫽锛�37鈩冩韩鑲�1灏忔檪銆�
4锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑诧紝鐢ㄥ惛姘寸礄鎷嶅共銆傞噸寰╂鎿嶄綔3娆°�傚鏋滅敤娲楁澘姗熸礂婊岋紝娲楁粚娆℃暩(sh霉)澧炲姞涓�娆°��
5锛� 姣忓瓟鍔犲叆80ul鐨勮Κ鍜岄張閰剁礌-HRP锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�30鍒嗛悩銆�
6锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑诧紝鐢ㄥ惛姘寸礄鎷嶅共銆傞噸寰╂鎿嶄綔3娆°�傚鏋滅敤娲楁澘姗熸礂婊岋紝娲楁粚娆℃暩(sh霉)澧炲姞涓�娆°��
7锛� 姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50ul锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�10鍒嗛悩銆傞伩鍏嶅厜鐓с��
8锛� 鍙栧嚭閰舵鏉匡紝杩呴�熷姞鍏�50ul绲傛娑诧紝鍔犲叆绲傛娑插悗鎳夌珛鍗虫脯瀹氱祼(ji茅)鏋溿��
9锛� 鍦�450nm娉㈤暦铏曟脯瀹氬悇瀛旂殑OD鍊笺��6铏熸婧栧搧浠ヤ笂鐨勭祼(ji茅)鏋滅偤闈炵窔鎬х殑锛屾牴鎿�(j霉)姝ゆ婧栨洸绶氱劇娉曞緱鍒扮殑绲�(ji茅)鏋溿��
澶ч紶澶氬反鑳篍LISA 妾㈡脯瑭﹀姂鐩�
瑭﹀姂鐩掓�ц兘
1. 闈堟晱搴︼細zui灏忕殑妾㈡脯婵冨害灏忎簬1铏熸婧栧搧銆傜█閲嬪害鐨勭窔鎬с�傛ǎ鍝佺窔鎬у洖姝歌垏闋愭湡婵冨害鐩搁棞绯绘暩(sh霉)R鍊肩偤0.990銆�
2. 鐗圭暟鎬э細涓嶈垏鍏跺畠绱拌優鍥犲瓙鍙嶆噳銆�
3. 閲嶅京鎬э細鏉垮収(n猫i)銆佹澘闁撹畩鐣扮郴鏁�(sh霉)鍧囧皬浜�10%銆�绲�(ji茅) 鏋� 鍒� 鏂� 鑸� 鍒� 鏋�
1銆佸剙鍣ㄥ�硷細浜庢尝闀�450nm鐨勯叾妯欏剙涓婅畝鍙栧悇瀛旂殑OD鍊�2銆佷互鍚稿厜搴D鍊肩偤绺卞潗妯欙紙Y锛夛紝鐩告噳鐨凞opamine妯欐簴鍝佹績搴︾偤姗潗妯欙紙X锛夛紝鍋氬緱鐩告噳鐨勬洸绶氾紝妯ｅ搧鐨凞opamine鍚噺鍙牴鎿�(j霉)鍏禣D鍊肩敱妯欐簴鏇茬窔鎻涚畻鍑虹浉鎳夌殑婵冨害銆�
3銆佹娓�艰寖鍦嶏細0-1600ng/L
4銆佹晱鎰熷害锛� 1.0 ng/L
Albumin, bovine, fraction V, cold alcohol isolation 鐗涜娓呯櫧铔嬬櫧 [9048-46-8] 1g
Albumin, bovine, fraction V, cold alcohol isolation 鐗涜娓呯櫧铔嬬櫧 [9048-46-8] 5g
Methyl red sodium salt 鐢插熀绱呴垑 [845-10-3] 100g
Albumin, bovine, fraction V, cold alcohol isolation 鐗涜娓呯櫧铔嬬櫧 [9048-46-8] 10g
Albumin, bovine, fraction V, heat shock isolation pH 7.0 鐗涜娓呯櫧铔嬬櫧 [9048-46-8] 100g
Naphthol AS-D acetate 钀橀厷AS-D-涔欓吀閰� [528-66-5] 250mg
Albumin, bovine Solution, 20 mg/ml 鐗涜娓呯櫧铔嬬櫧妯欐簴婧舵恫 / 1ml
Albumin, bovine Solution, 0.5mg/ml 鐗涜娓呯櫧铔嬬櫧妯欐簴婧舵恫 / 0.5ml
Alcian blue 8GX 闃跨埦鏂拌棈8GX [33864-99-2] 1g
Alcian blue 8GX 闃跨埦鏂拌棈8GX [33864-99-2] 5g
Alcohol dehydrogenase >300U/mg 涔欓唶鑴矮閰� [9031-72-5] 15KU
Alizarin 鑼滅礌 [72-48-0] 50g
Alizarin complexone 鑼滅礌绲″悎鎸囩ず鍔� [3952-78-1] 1g
Alizarin red 鑼滅礌绱� [130-22-3] 25g
Alcohol dehydrogenase >300U/mg 涔欓唶鑴矮閰� [9031-72-5] 75KU
Alendronate sodium, trihydrate 闃夸緰鑶﹂吀閳� [121268-17-5] 25g
Alizarin yellow GG 鑼滅礌榛� GG [584-42-9] 25g
Alizarin yellow GG 鑼滅礌榛� GG [584-42-9] 25g
Alizarin yellow R 鑼滅礌榛僐 [2243-76-7] 25g
5g
D-Biotin D-鐢熺墿绱� [58-85-5] 1g
D-Biotin D-鐢熺墿绱� [58-85-5] 5g
D-Allose D-闃挎礇绯� [2595-97-3] 25g
Alizarin yellow R 鑼滅礌榛僐 [2243-76-7] 25g
Alizarin yellow R , sodium salt 鑼滅礌榛僐閳夐菇 [1718-34-9] 25g
Alkali blue 6B 鍫胯棈 6B [30586-13-1] 25g
2024涓栫挵(hu谩n)鏈冦�愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�
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涓婁竴绡囷細鑱氭皑閰繚婧瑁滃彛闆荤啽鐔斿浣跨敤鏂规硶
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