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96T澶ч紶鍞炬恫閰窫LISA 妾㈡脯(c猫)瑭﹀姂鐩�
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澶ч紶鍞炬恫閰窫LISA 妾㈡脯(c猫)瑭﹀姂鐩�
鏈│鍔戝儏渚涚爺绌朵娇鐢� 妯�(bi膩o)鏈細琛�娓呮垨琛�婕�
瑭﹂(y脿n)鍘熺悊锛�
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2锛� 鍔犳ǎ鍣細5ul銆�10ul銆�50ul銆�100ul銆�200銆�500ul銆�1000ul銆�
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瀹夊叏鎬�
1锛� 閬垮厤鐩存帴鎺ヨЦ绲傛娑插拰搴曠墿A銆丅銆備竴鏃︽帴瑙稿埌閫欎簺娑查珨锛岃珛(q菒ng)鐩″揩鐢ㄦ按娌栨礂銆�
2锛� 瀵�(sh铆)椹�(y脿n)涓笉瑕佸悆鍠�銆佹娊鐓欐垨浣跨敤鍖栧鍝併��
3锛� 涓嶈鐢ㄥ槾鍚稿彇瑭﹀姂鐩掗噷鐨勪换浣曟垚浠姐��鎿嶄綔娉ㄦ剰浜嬮爡(xi脿ng)
1锛� 瑭﹀姂鎳�(y墨ng)鎸夋(bi膩o)绨借(shu艒)鏄庢浉(sh奴)鍎�(ch菙)瀛�锛屼娇鐢ㄥ墠鎭㈠京(f霉)鍒板婧�銆傜█绋�閬�(gu貌)鍚庣殑妯�(bi膩o)婧�(zh菙n)鍝佹噳(y墨ng)涓熸锛屼笉鍙繚瀛�銆�
2锛� 瀵�(sh铆)椹�(y脿n)涓笉鐢ㄧ殑鏉挎鎳�(y墨ng)绔嬪嵆鏀惧洖鍖呰琚嬩腑锛屽瘑灏佷繚瀛�锛屼互鍏嶈畩璩�(zh矛)銆�
3锛� 涓嶇敤鐨勫叾瀹冭│鍔戞噳(y墨ng)鍖呰濂芥垨钃嬪ソ銆備笉鍚屾壒铏�(h脿o)鐨勮│鍔戜笉瑕佹贩鐢�銆備繚璩�(zh矛)鍓嶄娇鐢ㄣ��
4锛� 浣跨敤涓�娆℃�х殑鍚搁牠浠ュ厤浜ゅ弶姹℃煋锛屽惛鍙栫祩姝㈡恫鍜屽簳鐗〢銆丅娑叉檪(sh铆)锛岄伩鍏嶄娇鐢ㄥ付閲戝爆閮ㄥ垎鐨勫姞妯ｅ櫒銆�
5锛� 浣跨敤骞插噲鐨勫鏂欏鍣ㄩ厤缃礂婊屾恫銆備娇鐢ㄥ墠鍏呭垎娣峰嫽瑭﹀姂鐩掗噷鐨勫悇绋垚浠藉強妯ｅ搧銆�
6锛� 娲楁粚閰舵(bi膩o)鏉挎檪(sh铆)鎳�(y墨ng)鍏呭垎鎷嶅共锛屼笉瑕佸皣鍚告按绱欑洿鎺ユ斁鍏ラ叾妯�(bi膩o)鍙嶆噳(y墨ng)瀛斾腑鍚告按銆�
7锛� 搴曠墿A鎳�(y墨ng)鎻櫦(f膩)锛岄伩鍏嶉暦(zh菐ng)鏅�(sh铆)闁撴墦闁�(k膩i)钃嬪瓙銆傚簳鐗〣灏�(du矛)鍏夋晱鎰�锛岄伩鍏嶉暦(zh菐ng)鏅�(sh铆)闁撴毚闇蹭簬鍏変笅銆傞伩鍏嶇敤鎵嬫帴瑙�锛屾湁姣�銆傚(sh铆)椹�(y脿n)瀹屾垚鍚庢噳(y墨ng)绔嬪嵆璁�鍙朞D鍊�銆�
8锛� 鍔犲叆瑭﹀姂鐨勯爢搴忔噳(y墨ng)涓�鑷达紝浠ヤ繚璀夋墍鏈夊弽鎳�(y墨ng)鏉垮瓟婧偛鐨勬檪(sh铆)闁撲竴妯�銆�
9锛� 鎸夌収瑾�(shu艒)鏄庢浉(sh奴)涓(bi膩o)鏄庣殑鏅�(sh铆)闁�銆佸姞娑茬殑閲忓強闋嗗簭閫�(j矛n)琛屾韩鑲叉搷浣�銆�澶ч紶鍞炬恫閰窫LISA 妾㈡脯(c猫)瑭﹀姂鐩�
妯ｅ搧鏀堕泦銆佽檿鐞嗗強淇濆瓨鏂规硶
1銆� 琛�娓�-----鎿嶄綔閬�(gu貌)绋嬩腑閬垮厤浠讳綍绱�(x矛)鑳炲埡婵�銆備娇鐢ㄤ笉鍚啽鍘熷拰鍏�(n猫i)姣掔礌鐨勮│绠�銆傛敹闆嗚娑插悗锛�1000×g闆㈠績10鍒嗛悩灏囪娓呭拰绱呯窗(x矛)鑳炶繀閫熷皬蹇冨湴鍒嗛洟銆�
2銆� 琛�婕�-----EDTA銆佹妾吀楣�銆佽倽绱犺婕垮彲鐢ㄤ簬妾㈡脯(c猫)銆�1000×g闆㈠績30鍒嗛悩鍘婚櫎椤嗙矑銆�
3銆� 绱�(x矛)鑳炰笂娓呮恫---1000×g闆㈠績10鍒嗛悩鍘婚櫎椤嗙矑鍜岃仛鍚堢墿銆�
4銆� 绲勭箶鍕绘伎-----灏囩祫绻斿姞鍏ラ仼閲忕敓鐞嗛菇姘存悧纰庛��1000×g闆㈠績10鍒嗛悩锛屽彇涓婃竻娑�
5銆� 淇濆瓨------濡傛灉妯ｅ搧涓嶇珛鍗充娇鐢�锛屾噳(y墨ng)灏囧叾鍒嗘垚灏忛儴鍒�-70 鈩冧繚瀛橈紝閬垮厤鍙嶅京(f霉)鍐峰噸銆傜洝鍙兘鐨勪笉瑕佷娇鐢ㄦ憾琛�鎴栭珮琛�鑴傝銆傚鏋滆娓呬腑澶ч噺椤嗙矑锛屾娓�(c猫)鍓嶅厛闆㈠績鎴栭亷(gu貌)婵�銆備笉瑕佸湪37鈩冩垨鏇撮珮鐨勬韩搴﹀姞鐔辫В鍑�銆傛噳(y墨ng)鍦ㄥ婧笅瑙ｅ噸骞剁⒑淇濇ǎ鍝佸潎鍕诲湴鍏呭垎瑙ｅ噸銆�瑭﹀姂鐨勬簴(zh菙n)鍌�
1锛� 妯�(bi膩o)婧�(zh菙n)鍝侊細妯�(bi膩o)婧�(zh菙n)鍝佺殑绯诲垪绋�閲�?xi臎)?y墨ng)鍦ㄥ(sh铆)椹�(y脿n)鏅�(sh铆)婧�(zh菙n)鍌�锛屼笉鑳藉劜(ch菙)瀛樸�傜█閲嬪墠灏囨(bi膩o)婧�(zh菙n)鍝佹尟钑╂贩鍕�銆傜█閲嬫瘮渚嬫寜涓嬭〃涓��(j矛n)琛岋細
800 mg/L 锛�6铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 鍘熷�嶆績搴︿笉鐢ㄧ█閲嬬洿鎺ュ姞鍏�50ul銆�
400 mg/L 锛�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨勫師鍊嶆(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
200 mg/L 锛�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
100 mg/L 锛�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
50 mg/L 锛�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
25 mg/L 锛�1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
0 mg/L 锛堢┖鐧藉皪(du矛)鐓э級 鍘熷婵冨害涓嶇敤绋�閲嬬洿鎺ュ姞鍏�50ul銆�2锛� 娲楁粚绶╂矕娑诧紙50×锛夌殑绋�閲�?zhu膩n)璧捍s姘�50鍊嶇█閲�銆�
鎿嶄綔姝ラ
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2锛� 鏍规摎(j霉)寰呮脯(c猫)妯ｅ搧鏁�(sh霉)閲忓姞涓婃(bi膩o)婧�(zh菙n)鍝佺殑鏁�(sh霉)閲忔焙瀹氭墍闇�鐨勬澘姊濇暩(sh霉)銆傛瘡鍊�(g猫)妯�(bi膩o)婧�(zh菙n)鍝佸拰绌虹櫧瀛斿缓璀板仛寰�(f霉)瀛�銆傛瘡鍊�(g猫)妯ｅ搧鏍规摎(j霉)鑷繁鐨勬暩(sh霉)閲忎締(l谩i)瀹�锛岃兘浣跨敤寰�(f霉)瀛旂殑鐩￠噺鍋氬京(f霉)瀛�銆傛(bi膩o)鏈敤妯�(bi膩o)鏈█閲嬫恫1锛�1绋�閲嬪悗鍔犲叆50ul浜庡弽鎳�(y墨ng)瀛斿収(n猫i)銆�
3锛� 鍔犲叆绋�閲嬪ソ鍚庣殑妯�(bi膩o)婧�(zh菙n)鍝�50ul浜庡弽鎳�(y墨ng)瀛�銆佸姞鍏ュ緟娓�(c猫)妯ｅ搧50ul浜庡弽鎳�(y墨ng)瀛斿収(n猫i)銆傜珛鍗冲姞鍏�50ul鐨勭敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨銆傝搵涓婅啘鏉�锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�1灏忔檪(sh铆)銆�
4锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊�(m菐n)娲楁粚娑�锛屾尟钑�30绉�锛岀敥鍘绘礂婊屾恫锛岀敤鍚告按绱欐媿骞�銆傞噸寰�(f霉)姝ゆ搷浣�3娆�銆傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆°��
5锛� 姣忓瓟鍔犲叆80ul鐨勮Κ鍜岄張閰剁礌-HRP锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�30鍒嗛悩銆�
6锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊�(m菐n)娲楁粚娑�锛屾尟钑�30绉掞紝鐢╁幓娲楁粚娑�锛岀敤鍚告按绱欐媿骞�銆傞噸寰�(f霉)姝ゆ搷浣�3娆°�傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆�銆�
7锛� 姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50ul锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�10鍒嗛悩銆傞伩鍏嶅厜鐓�銆�
8锛� 鍙栧嚭閰舵(bi膩o)鏉�锛岃繀閫熷姞鍏�50ul绲傛娑�锛屽姞鍏ョ祩姝㈡恫鍚庢噳(y墨ng)绔嬪嵆娓�(c猫)瀹氱祼(ji茅)鏋�銆�
9锛� 鍦�450nm娉㈤暦(zh菐ng)铏曟脯(c猫)瀹氬悇瀛旂殑OD鍊笺��6铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佷互涓婄殑绲�(ji茅)鏋滅偤闈炵窔鎬х殑锛屾牴鎿�(j霉)姝ゆ(bi膩o)婧�(zh菙n)鏇茬窔鐒�(w煤)娉曞緱鍒扮殑绲�(ji茅)鏋�銆�
澶ч紶鍞炬恫閰窫LISA 妾㈡脯(c猫)瑭﹀姂鐩�
瑭﹀姂鐩掓�ц兘
1. 闈堟晱搴︼細zui灏忕殑妾㈡脯(c猫)婵冨害灏忎簬1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝併�傜█閲嬪害鐨勭窔鎬�銆傛ǎ鍝佺窔鎬у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊肩偤0.990銆�
2. 鐗圭暟鎬э細涓嶈垏鍏跺畠绱�(x矛)鑳炲洜瀛愬弽鎳�(y墨ng)銆�
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1銆佸剙鍣ㄥ�硷細浜庢尝闀�(zh菐ng)450nm鐨勯叾妯�(bi膩o)鍎�涓婅畝鍙栧悇瀛旂殑OD鍊�2銆佷互鍚稿厜搴D鍊肩偤绺卞潗妯�(bi膩o)锛圷锛�锛岀浉鎳�(y墨ng)鐨凷A妯�(bi膩o)婧�(zh菙n)鍝佹績搴︾偤姗潗妯�(bi膩o)锛圶锛夛紝鍋氬緱鐩告噳(y墨ng)鐨勬洸绶�锛屾ǎ鍝佺殑SA鍚噺鍙牴鎿�(j霉)鍏禣D鍊肩敱妯�(bi膩o)婧�(zh菙n)鏇茬窔鎻涚畻鍑虹浉鎳�(y墨ng)鐨勬績搴︺��
3銆佹娓�(c猫)鍊艰寖鍦嶏細0-800mg/L
4銆佹晱鎰熷害锛� 1.0 mg/L
Ruthenium Red 閲曠磪 [11103-72-3] 100mg
Azure B 澶╅潚B [531-55-5] 25g
Azelaic acid 澹簩閰� [123-99-9] 250g
Aflatoxin G1 榛冩洸闇夋瘨绱燝1 [1165-39-5] 1mg
Aflatoxin G2 榛冩洸闇夋瘨绱燝2 [7241-98-7] 1mg
Aflatoxin M1 from Aspergillus flavus 榛冩洸闇夋瘨绱燤1 [6795-23-9] 1mg
Agarose ITM 鐡婅剛绯� ITM [9012-36-6] 25g
Allura red 瑾樻儜绱� [25956-17-6] 100g
Ethyl red 涔欏熀绱� [76058-33-8] 50g
BPDA 鑱�(li谩n)鑻簩閰� [2420-87-3] 100g
Agarose IIITM 鐡婅剛绯� IIITM [9012-36-6] 5g
Agarose IIITM 鐡婅剛绯� IIITM [9012-36-6] 25g
Agarose IVTM 鐡婅剛绯� IVTM [9012-36-6] 5g
BCA, disodium salt 2,2’-鑱�(li谩n)鍠瑰晧-4,4’-浜岀晶閰镐簩閳� [979-88-4] 25g
Benzil 鑱�(li谩n)鑻敳閰� [134-81-6] 25g
Agarose IVTM 鐡婅剛绯� IVTM [9012-36-6] 25g
Agarose B, Low EEO 鐡婅剛绯� [9012-36-6] 50g
Agarose B, Low EEO 鐡婅剛绯� [9012-36-6] 250g
Agarose H 鐡婅剛绯朒 [9012-36-6] 50g
Agarose H 鐡婅剛绯朒 [9012-36-6] 250g
Agarose, Low melting 浣庣啍榛�(di菐n)鐡婅剛绯� [9012-36-6] 5g
Agarose, Low melting 浣庣啍榛�(di菐n)鐡婅剛绯� [9012-36-6] 25g
Benzimidazole 鑻苟鍜攽 [51-17-2] 25g
Benzopurpurine 4B 鑻磪绱�4B [992-59-6] 25g
BAEE 鑻敳閰�-L-绮炬皑閰镐箼閰菇閰搁菇 [2645-08-1] 1g
Alcian yellow 闃垮埄鏂伴粌GXS锛堬級 [61968-76-1] 50g
Ala-Gln 涓欒胺浜岃偨 [39537-23-0] 1g
β-Alanine β-涓欐皑閰� [107-95-9] 100g
D-Alanine D-涓欐皑閰� [338-69-2] 100g
DL-Alanine DL-涓欐皑閰� [302-72-7] 500g
L-Alanine L-涓欐皑閰� [56-41-7] 100g
L-Alanine L-涓欐皑閰� [56-41-7] 500g
DL-Alaninol DL-姘ㄥ熀涓欓唶 [6168-72-5] 250ml
Albumin, from bovine Cohn fraction V pH 5.2 鐗涜娓呯櫧铔嬬櫧 [9048-46-8] 50g
Albumin, from bovine pH 7.0 Fatty free 鐒�(w煤)鑴傝偑閰哥墰琛�娓呯櫧铔嬬櫧 [9048-46-8] 50g
Albumin, from bovine Protease free 鐒�(w煤)铔嬬櫧閰剁墰琛�娓呯櫧铔嬬櫧 [9048-46-8] 50g
Albumin, from bovine pH 7.0 Low endotoxin 浣庡収(n猫i)姣掔礌鐗涜娓呯櫧铔嬬櫧 [9048-46-8] 50g
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