閱涚硸閭勫師閰剁浉浼艰泲鐧�1ELISA瑭﹀姂鐩掕(shu艒)鏄庢浉

2024骞�11鏈�19鏃� 16:36:41浜烘埃锛�64渚�(l谩i)婧愶細涓婃捣閭︽櫙瀵�(sh铆)妤�(y猫)鏈夐檺鍏徃

璩囨枡椤炲瀷	doc鏂囦欢	璩囨枡澶у皬	29184
涓嬭級娆℃暩(sh霉)	0	璩囨枡鍦栫墖	銆愰粸(di菐n)鎿婃煡鐪嬨€�
涓� 鍌� 浜�	涓婃捣閭︽櫙瀵�(sh铆)妤�(y猫)鏈夐檺鍏徃	闇€瑕佺⿳鍒�	0
闂�(gu膩n) 閸� 瑭�	閱涚硸閭勫師閰剁浉浼艰泲鐧�1ELISA瑭﹀姂鐩�,瑾�(shu艒)鏄庢浉

銆愯硣鏂欑啊(ji菐n)浠嬨€�

閱涚硸閭勫師閰剁浉浼艰泲鐧�1ELISA瑭﹀姂鐩掕(shu艒)鏄庢浉

妯�(bi膩o)鏈姹�:

1锛庢(bi膩o)鏈噰闆嗗悗鐩℃棭閫�(j矛n)琛屾彁鍙�锛屾彁鍙栨寜鐩搁棞(gu膩n)鏂囩嵒(xi脿n)閫�(j矛n)琛�锛屾彁鍙栧悗鎳�(y墨ng)鐩″揩閫�(j矛n)琛屽(sh铆)椹�(y脿n)銆傝嫢涓嶈兘棣笂閫�(j矛n)琛岃│椹�(y脿n)锛屽彲灏囨(bi膩o)鏈斁浜�-20鈩冧繚瀛�锛屼絾鎳�(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺

2锛庝笉鑳芥娓�(c猫)鍚玁aN3鐨勬ǎ鍝�锛屽洜NaN3鎶戝埗杈ｆ牴閬�(gu貌)姘у寲鐗╅叾鐨勶紙HRP锛夋椿鎬�銆�

妯ｆ湰铏曠悊鍙婅姹傦細

1.琛€娓咃細鍏ㄨ妯�(bi膩o)鏈珛(q菒ng)浜庡婧斁缃�2灏忔檪(sh铆)鎴�4鈩冮亷(gu貌)澶滃悗浜�1000g闆㈠績20鍒嗛悩锛屽彇涓婃竻鍗冲彲妾㈡脯(c猫)锛屾垨?q奴)顣�?bi膩o)鏈斁浜�-20鈩冩垨-80鈩冧繚瀛橈紝浣嗘噳(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�

2.琛€婕匡細鍙敤EDTA鎴栬倽绱犱綔鐐烘姉鍑濆姂锛屾(bi膩o)鏈噰闆嗗悗30鍒嗛悩鍏�(n猫i)浜�2 - 8掳C 1000g闆㈠績20鍒嗛悩锛屾垨?q奴)顣�?bi膩o)鏈斁浜�-20鈩冩垨-80鈩冧繚瀛�锛屼絾鎳�(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�

3.绲勭箶鍕绘伎锛氱敤闋�(y霉)鍐风殑PBS (0.01M, pH=7.4)娌栨礂绲勭箶锛屽幓闄ゆ畼鐣欒娑诧紙鍕绘伎涓瑙ｇ殑绱呯窗(x矛)鑳炴渻(hu矛)褰遍熆娓�(c猫)閲忕祼(ji茅)鏋滐級锛岀ū閲嶅悗灏囩祫绻斿壀纰�銆傚皣鍓鐨勭祫绻旇垏灏�(du矛)鎳�(y墨ng)楂旂⿳鐨凱BS锛堜竴鑸寜1:9鐨勯噸閲忛珨绌嶆瘮锛屾瘮濡�1g鐨勭祫绻旀ǎ鍝佸皪(du矛)鎳�(y墨ng)9mL鐨凱BS锛屽叿楂旈珨绌嶅彲鏍规摎(j霉)瀵�(sh铆)椹�(y脿n)闇€瑕侀仼鐣�(d膩ng)瑾�(di脿o)鏁达紝骞跺仛濂借閷�銆傛帹钖﹀湪PBS涓姞鍏ヨ泲鐧介叾鎶戝埗鍔戯級鍔犲叆鐜荤拑鍕绘伎鍣ㄤ腑锛屼簬鍐颁笂鍏呭垎鐮旂（銆傜偤浜嗛€�(j矛n)涓€姝ヨ瑙ｇ祫绻旂窗(x矛)鑳�锛屽彲浠ュ皪(du矛)鍕绘伎娑查€�(j矛n)琛岃秴鑱茬牬纰�锛屾垨鍙嶅京(f霉)鍑嶈瀺銆傚皣鍕绘伎娑蹭簬5000脳g闆㈠績5~10鍒嗛悩锛屽彇涓婃竻妾㈡脯(c猫)銆�

4.绱�(x矛)鑳炲煿椁�(y菐ng)鐗╀笂娓呮垨鍏跺畠鐢熺墿妯�(bi膩o)鏈細1000g闆㈠績20鍒嗛悩锛屽彇涓婃竻鍗冲彲妾㈡脯(c猫)锛屾垨?q奴)顣�?bi膩o)鏈斁浜�-20鈩冩垨-80鈩冧繚瀛橈紝浣嗘噳(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�

瑭﹀姂鐩掔祫鎴� :

1銆�30鍊嶆績绺礂婊屾恫 20ml脳1鐡� 7 绲傛娑� 6ml脳1鐡�

2銆�閰舵(bi膩o)瑭﹀姂 6ml脳1鐡� 8 妯�(bi膩o)婧�(zh菙n)鍝侊紙160pg/ml锛� 0.5ml脳1鐡�

3銆�閰舵(bi膩o)鍖呰鏉� 12瀛斆�8姊� 9 妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫 1.5ml脳1鐡�

4銆�妯ｅ搧绋€閲嬫恫 6ml脳1鐡� 10 瑾�(shu艒)鏄庢浉 1浠�

5銆�椤壊鍔�A娑� 6ml脳1鐡� 11 灏佹澘鑶� 2寮�

6銆�椤壊鍔�B娑� 6ml脳1/鐡� 12 瀵嗗皝琚� 1鍊�(g猫)

瀵�(sh铆)椹�(y脿n)鍘熺悊:

鏈│鍔戠洅鎳�(y墨ng)鐢ㄩ洐鎶楅珨澶惧績娉曟脯(c猫)瀹氭(bi膩o)鏈腑琛€娓呯礌/琛€娓呰兒(ST)姘村钩銆傜敤绱斿寲鐨勮娓呯礌/琛€娓呰兒(ST)鎶楅珨鍖呰寰瓟鏉匡紝鍒舵垚鍥虹浉鎶楅珨锛屽線鍖呰鍠姉鐨勫井瀛斾腑渚濇鍔犲叆琛€娓呯礌/琛€娓呰兒(ST)锛屽啀鑸嘓RP妯�(bi膩o)瑷樼殑琛€娓呯礌/琛€娓呰兒(ST)鎶楅珨绲�(ji茅)鍚�锛屽舰鎴愭姉楂�-鎶楀師-閰舵(bi膩o)鎶楅珨寰�(f霉)鍚堢墿锛岀稉(j墨ng)閬�(gu貌)寰�di娲楁粚鍚庡姞搴曠墿TMB椤壊銆俆MB鍦℉RP閰禨鐨勫偓鍖栦笅杞�(zhu菐n)鍖栨垚钘�(l谩n)鑹�锛屽苟鍦ㄩ吀鐨勪綔鐢ㄤ笅杞�(zhu菐n)鍖栨垚鏈€绲傜殑榛冭壊銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑鐨勮娓呯礌/琛€娓呰兒(ST)鍛堟鐩搁棞(gu膩n)銆傜敤閰舵(bi膩o)鍎€鍦�450nm娉㈤暦(zh菐ng)涓嬫脯(c猫)瀹氬惛鍏夊害锛圤D鍊硷級锛岄€氶亷(gu貌)妯�(bi膩o)婧�(zh菙n)鏇茬窔瑷�(j矛)绠楁ǎ鍝佷腑琛€娓呯礌/琛€娓呰兒(ST)婵冨害銆�

瑭﹀姂鐩掓€ц兘锛�

1. 闈堟晱搴︼細鏈€灏忕殑妾㈡脯(c猫)婵冨害灏忎簬1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝併€傜█閲嬪害鐨勭窔鎬с€傛ǎ鍝佺窔鎬у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊肩偤0.990銆�

2. 鐗圭暟鎬э細涓嶈垏鍏跺畠绱�(x矛)鑳炲洜瀛愬弽鎳�(y墨ng)銆�

3. 閲嶅京(f霉)鎬э細鏉垮収(n猫i)銆佹澘闁撹畩鐣扮郴鏁�(sh霉)鍧囧皬浜�10%銆�

鎿嶄綔姝ラ锛�

1. 妯�(bi膩o)婧�(zh菙n)鍝佺殑绋€閲嬶細鏈│鍔戠洅鎻愪緵鍘熷€嶆(bi膩o)婧�(zh菙n)鍝佷竴鏀紝鐢ㄦ埗鍙寜鐓т笅鍒楀湒琛ㄥ湪灏忚│绠′腑閫�(j矛n)琛岀█閲�銆�

80pg/ml	5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝�	150渭l鐨勫師鍊嶆(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
40pg/ml	4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝�	150渭l鐨�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
20pg/ml	3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝�	150渭l鐨�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
10pg/ml	2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝�	150渭l鐨�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
5pg/ml	1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝�	150渭l鐨�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫

2. 鍔犳ǎ锛氬垎鍒ヨō(sh猫)绌虹櫧瀛旓紙绌虹櫧灏�(du矛)鐓у瓟涓嶅姞妯ｅ搧鍙婇叾妯�(bi膩o)瑭﹀姂锛屽叾浣欏悇姝ユ搷浣滅浉鍚岋級銆佹(bi膩o)婧�(zh菙n)瀛�銆佸緟娓�(c猫)妯ｅ搧瀛�銆傚湪閰舵(bi膩o)鍖呰鏉夸笂妯�(bi膩o)婧�(zh菙n)鍝佹簴(zh菙n)纰哄姞妯�50渭l锛屽緟娓�(c猫)妯ｅ搧瀛斾腑鍏堝姞妯ｅ搧绋€閲嬫恫40渭l锛岀劧鍚庡啀鍔犲緟娓�(c猫)妯ｅ搧10渭l锛堟ǎ鍝佹渶绲傜█閲嬪害鐐�5鍊嶏級銆傚姞妯ｅ皣妯ｅ搧鍔犱簬閰舵(bi膩o)鏉垮瓟搴曢儴锛岀洝閲忎笉瑙稿強瀛斿锛岃紩杓曟檭鍕�(d貌ng)娣峰嫽銆�

3. 婧偛锛氱敤灏佹澘鑶滃皝鏉垮悗缃�37鈩冩韩鑲�30鍒嗛悩銆�

4. 閰嶆恫锛氬皣30鍊嶆績绺礂婊屾恫鐢ㄨ捀椁炬按30鍊嶇█閲嬪悗鍌欑敤

5. 娲楁粚锛氬皬蹇冩彮鎺夊皝鏉胯啘锛屾鍘绘恫楂�锛岀敥骞�锛屾瘡瀛斿姞婊挎礂婊屾恫锛岄潨缃�30绉掑悗妫勫幓锛屽姝ら噸寰�(f霉)5娆�锛屾媿骞�銆�

6. 鍔犻叾锛氭瘡瀛斿姞鍏ラ叾妯�(bi膩o)瑭﹀姂50渭l锛岀┖鐧藉瓟闄ゅ銆�

7. 婧偛锛氭搷浣滃悓3銆�

8. 娲楁粚锛氭搷浣滃悓5銆�

9. 椤壊锛氭瘡瀛斿厛鍔犲叆椤壊鍔�A50渭l锛屽啀鍔犲叆椤壊鍔態50渭l锛岃紩杓曢渿钑╂贩鍕�锛�37鈩冮伩鍏夐’鑹�10鍒嗛悩.

10. 绲傛锛氭瘡瀛斿姞绲傛娑�50渭l锛岀祩姝㈠弽鎳�(y墨ng)锛堟鏅�(sh铆)钘�(l谩n)鑹茬珛杞�(zhu菐n)榛冭壊锛�銆�

11. 娓�(c猫)瀹氾細浠ョ┖鐧藉瓟瑾�(di脿o)闆讹紝450nm娉㈤暦(zh菐ng)渚濆簭娓�(c猫)閲忓悇瀛旂殑鍚稿厜搴︼紙OD鍊硷級銆� 娓�(c猫)瀹氭噳(y墨ng)鍦ㄥ姞绲傛娑插悗15鍒嗛悩浠ュ収(n猫i)閫�(j矛n)琛�銆�

娉ㄦ剰浜嬮爡(xi脿ng)锛�

1锛庤│鍔戠洅寰炲喎钘忕挵(hu谩n)澧冧腑鍙栧嚭鎳�(y墨ng)鍦ㄥ婧钩琛�15-30鍒嗛悩鍚庢柟鍙娇鐢�锛岄叾妯�(bi膩o)鍖呰鏉块枊灏佸悗濡傛湭鐢ㄥ畬锛屾澘姊濇噳(y墨ng)瑁濆叆瀵嗗皝琚嬩腑淇濆瓨銆�

2锛庢績娲楁粚娑插彲鑳芥渻(hu矛)鏈夌祼(ji茅)鏅舵瀽鍑�锛岀█閲嬫檪(sh铆)鍙湪姘存荡涓姞婧姪婧�锛屾礂婊屾檪(sh铆)涓嶅奖闊跨祼(ji茅)鏋�銆�

3锛庡悇姝ュ姞妯ｅ潎鎳�(y墨ng)浣跨敤鍔犳ǎ鍣紝骞剁稉(j墨ng)甯告牎灏�(du矛)鍏舵簴(zh菙n)纰烘€�锛屼互閬垮厤瑭﹂(y脿n)瑾ゅ樊銆備竴娆″姞妯ｆ檪(sh铆)闁撴渶濂芥帶鍒跺湪5鍒嗛悩鍏�(n猫i)锛屽妯�(bi膩o)鏈暩(sh霉)閲忓锛屾帹钖︿娇鐢ㄦ帓妲嶅姞妯�銆�

4锛� 璜�(q菒ng)姣忔娓�(c猫)瀹氱殑鍚屾檪(sh铆)鍋氭(bi膩o)婧�(zh菙n)鏇茬窔锛屾渶濂藉仛寰�(f霉)瀛�銆傚妯�(bi膩o)鏈腑寰呮脯(c猫)鐗╄唱(zh矛)鍚噺閬�(gu貌)楂橈紙妯ｆ湰OD鍊煎ぇ浜庢(bi膩o)婧�(zh菙n)鍝佸瓟绗竴瀛旂殑OD鍊硷級锛岃珛(q菒ng)鍏堢敤妯ｅ搧绋€閲嬫恫绋€閲嬩竴瀹氬€嶆暩(sh霉)锛坣鍊嶏級鍚庡啀娓�(c猫)瀹�锛岃▓(j矛)绠楁檪(sh铆)璜�(q菒ng)鏈€鍚庝箻浠ョ附绋€閲嬪€嶆暩(sh霉)锛埫梟脳5锛�銆�

5锛� 灏佹澘鑶滃彧闄愪竴娆℃€т娇鐢�锛屼互閬垮厤浜ゅ弶姹℃煋銆�

6锛庡簳鐗╄珛(q菒ng)閬垮厜淇濆瓨銆�

7锛庡毚(y谩n)鏍兼寜鐓ц(shu艒)鏄庢浉鐨勬搷浣滈€�(j矛n)琛�锛岃│椹�(y脿n)绲�(ji茅)鏋滃垽瀹氬繀闋堜互閰舵(bi膩o)鍎€璁€鏁�(sh霉)鐐烘簴(zh菙n).

8锛庢墍鏈夋ǎ鍝侊紝娲楁粚娑插拰鍚勭ó寤㈡鐗╅兘鎳�(y墨ng)鎸夊偝鏌撶墿铏曠悊銆�

9锛庢湰瑭﹀姂涓嶅悓鎵硅櫉(h脿o)绲勫垎涓嶅緱娣风敤銆�

涓婃捣閭︽櫙瀵�(sh铆)妤�(y猫)鏈夐檺鍏徃浣滆€�

閫�(j矛n)鍏ュ睍鑷�(t谩i)鍦ㄧ窔鐣欒█

2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡€(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�

div6鏈�3鏃�锛�2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡€(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆戜簬涓婃捣涓ㄥ湅(gu贸)瀹舵渻(hu矛)灞曚腑[瑭崇窗(x矛)]

涓婁竴绡囷細澶ч紶toll妯ｅ彈楂�3锛圱LR3锛塃LISA瑭﹀姂鐩掓ǎ鏈(shu艒)鏄�

涓嬩竴绡囷細澶ч紶鐧戒粙绱�1伪锛圛L-1伪锛塃LISA鍒嗘瀽妾㈡脯(c猫)瑭﹀姂鐩掕│椹�(y脿n)鍘熺悊

鐗堟瑠(qu谩n)鑸囧厤璨�(z茅)鑱叉槑

鍑℃湰缍�(w菐ng)娉ㄦ槑"渚�(l谩i)婧愶細鐠�(hu谩n)淇濆湪绶�"鐨勬墍鏈変綔鍝�锛岀増娆�(qu谩n)鍧囧爆浜庣挵(hu谩n)淇濆湪绶�锛岃綁(zhu菐n)杓夎珛(q菒ng)蹇呴爤娉ㄦ槑鐠�(hu谩n)淇濆湪绶�锛宧ttp://www.hezebar.com銆傞仌鍙嶈€呮湰缍�(w菐ng)灏囪拷绌剁浉闂�(gu膩n)娉曞緥璨�(z茅)浠汇€�
浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛�(sh霉)鏂囩珷銆佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹�锛屽娑夊強渚垫瑠(qu谩n)銆侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂(d膩n)璨�(z茅)浠�锛屾湰缍�(w菐ng)鏈夋瑠(qu谩n)鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨�(z茅)浠汇€�
鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締(l谩i)婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭�锛屽苟涓嶄唬琛ㄦ湰缍�(w菐ng)璐婂悓鍏惰榛�(di菐n)鎴栬瓑瀵�(sh铆)鍏跺収(n猫i)瀹圭殑鐪熷(sh铆)鎬�锛屼笉鎵挎摂(d膩n)姝ら浣滃搧渚垫瑠(qu谩n)琛岀偤鐨勭洿鎺ヨ铂(z茅)浠诲強閫ｅ付璨�(z茅)浠�銆傚叾浠栧獟楂�銆佺恫(w菐ng)绔欐垨鍊�(g猫)浜哄緸鏈恫(w菐ng)杞�(zhu菐n)杓夋檪(sh铆)锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締(l谩i)婧�锛屽苟鑷矤(f霉)鐗堟瑠(qu谩n)绛夋硶寰嬭铂(z茅)浠�銆�
濡傛秹鍙婁綔鍝佸収(n猫i)瀹�銆佺増娆�(qu谩n)绛夊晱(w猫n)椤岋紝璜�(q菒ng)?ji膩n)璋ч珜閽抣(f膩)琛ㄤ箣鏃ヨ捣涓€鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯伙紝鍚﹀墖瑕栫偤鏀炬鐩搁棞(gu膩n)娆�(qu谩n)鍒�銆�

九一国产精品_日本免费不卡一区二区_在线a毛片_久久在草_国产一区二区影视_亚洲国产美女视频

閱涚硸閭勫師閰剁浉浼艰泲鐧�1ELISA瑭﹀姂鐩掕(shu艒)鏄庢浉

2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡€(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�

鐗堟瑠(qu谩n)鑸囧厤璨�(z茅)鑱叉槑